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Ppp1r15a antibody (A02394-4) performs well in ELISA, Flow Cytometry, WB and shows reactivity with Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of ANA-1 cells using anti-GADD34/Ppp1r15a antibody (A02394-4).
Overlay histogram showing ANA-1 cells stained with A02394-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GADD34/Ppp1r15a Antibody (A02394-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing ANA-1 cells stained with A02394-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GADD34/Ppp1r15a Antibody (A02394-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-GADD34/Ppp1r15a Antibody Picoband®
Ppp1r15a antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse | Rabbit | ELISA, Flow Cytometry, WB |
Tested Applications
Ppp1r15a antibody was positively detected by WB in:
mouse stomach tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Ppp1r15a antibody was positively detected by FC in:
ANA-1 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Mouse
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Mouse
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Product Information
Product Name
Anti-GADD34/Ppp1r15a Antibody Picoband®
View all PPP1R15A Antibodies
View all PPP1R15A Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse GADD34/Ppp1r15a recombinant protein (Position: R14-R572).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
100 kDa
Calculated Molecular Weight
71.8 kDa
Background of Ppp1r15a
Protein phosphatase 1 regulatory subunit 15A also known as growth arrest and DNA damage-inducible protein GADD34 is a protein that in humans is encoded by the PPP1R15A gene. Enables protein phosphatase binding activity and protein phosphatase regulator activity. Involved in several processes, including peptidyl-serine dephosphorylation; regulation of gene expression; and regulation of protein modification process. Acts upstream of or within endoplasmic reticulum unfolded protein response. Predicted to be located in several cellular components, including Golgi apparatus; cytosol; and mitochondrion. Predicted to be part of protein phosphatase type 1 complex. Predicted to be active in endoplasmic reticulum. Is expressed in several structures, including central nervous system; genitourinary system; gut; hemolymphoid system gland; and liver. Used to study thalassemia. Orthologous to human PPP1R15A (protein phosphatase 1 regulatory subunit 15A).
Cite This Product
Anti-GADD34/Ppp1r15a Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02394-4)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
67%
Low homology
Dog
51%
Low homology
Zebrafish
16%
Low homology
Drosophila
10%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Ppp1r15a antibody
Flow Cytometry (FC) protocol for Ppp1r15a antibody
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Product Promise
We promise Anti-GADD34/Ppp1r15a Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A02394-4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 16, 2026