Anti-Galectin 10/CLC Antibody Picoband™

Product Name

Anti-Galectin 10/CLC Antibody Picoband™

View all Galectin-10 Antibodies

SKU/Catalog Number

A01350

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Galectin 10/CLC Antibody Picoband™ catalog # A01350. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Galectin 10/CLC Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01350)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human Galectin 10 recombinant protein (Position: S2-R142).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01350 is reactive to CLC in Human

Applications

A01350 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

16 kDa

Calculated molecular weight

16.453kDa

Background of Galectin-10

Eosinophil lysophospholipase is an enzyme that in humans is encoded by the CLC gene. Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene is a lysophospholipase expressed in eosinophils and basophils. It hydrolyzes lysophosphatidylcholine to glycerophosphocholine and a free fatty acid. This protein may possess carbohydrate or IgE-binding activities. It is both structurally and functionally related to the galectin family of beta-galactoside binding proteins. It may be associated with inflammation and some myeloid leukemias.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Galectin 10 using anti-Galectin 10 antibody (A01350).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: human PANC-1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin 10 antigen affinity purified polyclonal antibody (Catalog # A01350) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galectin 10 at approximately 16KD. The expected band size for Galectin 10 is at 16KD.

Gene/Protein Information For CLC (Source: Uniprot.org, NCBI)

Alternative Names

Charcot-Leyden crystal proteinLGALS10A; CLC; EC 3.1.1.5; eosinophil lysophospholipase; GAL10; Gal-10; galectin 10; Galectin10; Galectin-10; LGALS10; LPPL_HUMAN; Lysolecithin acylhydrolase; MGC149659 CLC GAL10, Gal-10, LGALS10, LGALS10A, LPPL_HUMAN Charcot-Leyden crystal galectin galectin-10|Charcot-Leyden crystal protein|eosinophil lysophospholipase|lectin, galactoside-binding, soluble, 10|lysolecithin acylhydrolase

For more info on CLC, check out the CLC Infographic

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In this infographic, you will see the following information for CLC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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