|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-SLC9A2 Antibody|
|Description||Rabbit IgG polyclonal antibody for Sodium/hydrogen exchanger 2(SLC9A2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-SLC9A2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2219)|
|Replacement Item||This antibody may replace the following items: sc-22928|sc-30167 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human SLC9A2(666-684aa RYLSLPKNTKLPEKLQKRR), different from the related rat and mouse sequences by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Sodium/hydrogen exchanger 2|
|Molecular Weight||91520 MW|
|Protein Function||Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Seems to play an important role in colonic sodium absorption.|
|Tissue Specificity||Expressed in skeletal muscle, colon and kidney. Lower levels in the testis, prostate, ovary, and small intestine.|
|Sequence Similarities||Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.|
|Subcellular Localization||Membrane; Multi-pass membrane protein.|
|Alternative Names||Sodium/hydrogen exchanger 2;Na(+)/H(+) exchanger 2;NHE-2;Solute carrier family 9 member 2;SLC9A2;NHE2;|
Background for Sodium/hydrogen exchanger 2
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-SLC9A2 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Rat, Human, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-SLC9A2 Antibody Images
Click the images to enlarge.
Lane 1: Rat Skeletal Musle Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
IHC(P): Human Lung Cancer Tissue
IHC(P): Rat Kidney Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,