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Product Info Summary
| SKU: | M01868 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-GAP43 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01868
BM4473 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-GAP43 Rabbit Monoclonal Antibody catalog # M01868. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GAP43 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01868)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FOG-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human GAP43
Reactive Species
M01868 is reactive to GAP43 in Human, Mouse, Rat
Observed Molecular Weight
48, 54 kDa
Calculated molecular weight
24.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01868 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:10000-1:50000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:30
FC 1:20
Positive Control
WB: SH-SY5Y cell
IHC: human stomach tissue
Validation Images & Assay Conditions
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Western blot analysis of GAP43 expression in SH-SY5Y cell lysate.
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Immunohistochemical analysis of paraffin-embedded human stomach, using GAP43 Antibody.
Specific Publications For Anti-GAP43 Rabbit Monoclonal Antibody (M01868)
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4 Customer Q&As for Anti-GAP43 Rabbit Monoclonal Antibody
Question
Our team were content with the WB result of your anti-GAP43 Rabbit Monoclonal antibody. However we have been able to see positive staining in brain cell membrane using this antibody. Is that expected? Could you tell me where is GAP43 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
According to literature, brain does express GAP43. Generally GAP43 expresses in cell membrane. Regarding which tissues have GAP43 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Brain cortex, and Subthalamic nucleus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-04-09
Question
We are currently using anti-GAP43 Rabbit Monoclonal antibody M01868 for mouse tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
The anti-GAP43 Rabbit Monoclonal antibody (M01868) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-13
Question
We ordered your anti-GAP43 Rabbit Monoclonal antibody for IF on brain in a previous project. I am using human, and We intend to use the antibody for WB next. I am looking for examining brain as well as brain cortex subthalamic nucleus in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2018-05-30
Answer
I looked at the website and datasheets of our anti-GAP43 Rabbit Monoclonal antibody and it seems that M01868 has been validated on human in both IF and WB. Thus M01868 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-05-30
Question
We have been able to see staining in mouse brain. Are there any suggestions? Is anti-GAP43 Rabbit Monoclonal antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2017-07-07
Answer
Based on literature brain does express GAP43. Based on Uniprot.org, GAP43 is expressed in anterior cingulate cortex, brain cortex subthalamic nucleus, brain, among other tissues. Regarding which tissues have GAP43 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Brain cortex, and Subthalamic nucleus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-07-07
