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- Table of Contents
GATA3 antibody (PB9199) performs well in WB and shows reactivity with Human, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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Western blot analysis of GATA3 using anti-GATA3 antibody (PB9199).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human GATA3 protein 0.5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA3 antigen affinity purified polyclonal antibody (Catalog # PB9199) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATA3 at approximately 39 kDa. The expected band size for GATA3 is at 39 kDa.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human GATA3 protein 0.5 ng.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA3 antigen affinity purified polyclonal antibody (Catalog # PB9199) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATA3 at approximately 39 kDa. The expected band size for GATA3 is at 39 kDa.
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Anti-GATA3 Antibody Picoband®
GATA3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Rat | Rabbit | WB |
Tested Applications
GATA3 antibody was positively detected by WB in:
rat brain tissue, human MCF-7 whole cell, human HepG2 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
Rat
Tested
Human
Tested
Rabbit
Predicted
Monkey
Predicted
Bovine
Predicted
Sheep
Predicted
Mouse
Predicted
Product Information
Product Name
Anti-GATA3 Antibody Picoband®
View all GATA3 Antibodies
View all GATA3 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human GATA3 recombinant protein (Position: M1-E197). Human GATA3 shares 95% amino acid (aa) sequence identity with mouse GATA3.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
48 kDa
Calculated Molecular Weight
47.9 kDa
Background of GATA3
Trans-acting T-cell-specific transcription factor GATA-3, also named HDR or GATA3, is a protein that in humans is encoded by the GATA3 gene. It is mapped to 10p14. This gene belongs to the GATA family of transcription factors. It regulates luminal epithelial cell differentiation in the mammary gland. The protein contains two GATA-type zinc fingers, it is an important regulator of T cell development and plays an important role in endothelial cell biology. GATA3 has been shown to promote the secretion of IL-4, IL-5, and IL-13 from Th2 cells, and induces the differentiation of Th0 cells towards this T cell subtype while suppressing their differentiation towards Th1 cells.
Cite This Product
Anti-GATA3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9199)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Rabbit
98%
High Homology
Monkey
98%
High Homology
Cow
96%
High Homology
Sheep
96%
High Homology
Mouse
95%
High Homology
Rat
95%
High Homology
Zebrafish
74%
Moderate Homology
Drosophila
26%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for GATA3 antibody
Publications
Application:
Species:
Regulatory effects of miRNA-126 on Th cell differentiation and cytokine expression in allergic rhinitis
Published: 2022-08-08
Journal: CELLULAR SIGNALLING
Application: WB
Impact Factor: 4.85
Species: Rat
PMID: 35953026
Impaired TNF/TNFR2 signaling enhances Th2 and Th17 polarization and aggravates allergic airway inflammation
Published: 2017-09-01
Journal: AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
Application: WB
Impact Factor: 4.28
Species: Mouse
PMID: 28619762
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Product Promise
We promise Anti-GATA3 Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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PB9199
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026