Anti-GATA4 Monoclonal Antibody

Product Name

Anti-GATA4 Monoclonal Antibody

View all GATA-4 Antibodies

SKU/Catalog Number

M00499

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-GATA4 Monoclonal Antibody (Catalog # M00499). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-GATA4 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00499)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: 10D8.B10.D4

Isotype

IgG1 kappa

Immunogen

GATA4 protein A purified antibody was prepared in mice by repeated immunizations with a recombinant protein corresponding to the first 100 amino acids of human GATA4 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00499 is reactive to GATA4 in Human

Applications

M00499 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

44.565kDa

Background of GATA-4

GATA4, also know as GATA-binding factor 4, acts in cooperation with NKX2-5 as a transcriptional activator of ANF. It binds to the consensus sequence 5'-AGATAG-3' on DNA. GATA4 is critical for the enlargement of cardiac myocytes. Mutations in GATA4 have been linked with congenital heart malfunctions and malformations. Anti-GATA4 antibody is ideal for researchers interested in developmental biology, stem cell research, cell growth and cancer research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:2000 - 1:10,000
WB: 1:500 - 1:1000

Validation Images & Assay Conditions

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Western blot analysis of GATA4 expression in CCRF-CEM cell lysates (lane 1). GATA4 at 44.5KD was detected using mouse anti-GATA4 protein A purified monoclonal antibody (Catalog # M00499) at 1:500. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1001).

Gene/Protein Information For GATA4 (Source: Uniprot.org, NCBI)

Alternative Names

GATA binding protein 4; GATA4; GATA-4; GATA-binding factor 4; GATA-binding protein 4; MGC126629; transcription factor GATA-4 GATA4 ASD2, TACHD, TOF, VSD1 GATA binding protein 4 transcription factor GATA-4|GATA-binding factor 4

For more info on GATA4, check out the GATA4 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GATA4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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