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Product Info Summary
| SKU: | A01356-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-GDF-5 Antibody
SKU/Catalog Number
A01356-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-GDF-5 Antibody catalog # A01356-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GDF-5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01356-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human GDF5. AA range:361-410
Reactive Species
A01356-1 is reactive to GDF5 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
55.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01356-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC: 1:100-1:300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of 293 cells using GDF-5 Polyclonal Antibody. Secondary antibody was diluted at 1:20000
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Western blot analysis of lysate from 293 cells, using GDF5 Antibody.
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Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100
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Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100
Specific Publications For Anti-GDF-5 Antibody (A01356-1)
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Customer Q&As
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7 Customer Q&As for Anti-GDF-5 Antibody
Question
See attached the WB image, lot number and protocol we used for saliva-secreting gland using anti-GDF-5 antibody A01356-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-24
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for saliva-secreting gland using anti-GDF-5 antibody A01356-1. Let me know if you need anything else.
K. Patel
Verified customer
Asked: 2019-01-22
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-22
Question
Is this A01356-1 anti-GDF-5 antibody reactive to the isotypes of GDF5?
Verified Customer
Verified customer
Asked: 2019-01-18
Answer
The immunogen of A01356-1 anti-GDF-5 antibody is Synthesized peptide derived from the Internal region of human GDF-5. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-01-18
Question
My question regarding product A01356-1, anti-GDF-5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-09-14
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01356-1 anti-GDF-5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-09-14
Question
Will anti-GDF-5 antibody A01356-1 work for IHC with saliva-secreting gland?
E. Johnson
Verified customer
Asked: 2017-11-17
Answer
According to the expression profile of saliva-secreting gland, GDF5 is highly expressed in saliva-secreting gland. So, it is likely that anti-GDF-5 antibody A01356-1 will work for IHC with saliva-secreting gland.
Boster Scientific Support
Answered: 2017-11-17
Question
We are currently using anti-GDF-5 antibody A01356-1 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
E. Baker
Verified customer
Asked: 2016-05-31
Answer
The anti-GDF-5 antibody (A01356-1) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-05-31
Question
We are interested in to test anti-GDF-5 antibody A01356-1 on mouse saliva-secreting gland for research purposes, then I may be interested in using anti-GDF-5 antibody A01356-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Jackson
Verified customer
Asked: 2013-10-23
Answer
The products we sell, including anti-GDF-5 antibody A01356-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-10-23
