Anti-GDF-5 Antibody

GDF-5/BMP-14 antibody

Boster Bio Anti-GDF-5 Antibody catalog # A01356-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A01356-1
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-GDF-5 Antibody

View all GDF-5/BMP-14 Antibodies

SKU/Catalog Number

A01356-1

Size

100μl

Form

Liquid

Description

Boster Bio Anti-GDF-5 Antibody catalog # A01356-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GDF-5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01356-1)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

The antiserum was produced against synthesized peptide derived from the Internal region of human GDF5 at amino acid range 361-410

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A01356-1 is reactive to GDF5 in Human, Mouse, Rat

Applications

A01356-1 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

55.411kDa

Background of GDF-5/BMP-14

eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with eIF3, mutually exclusive with EIF4A1 or EIFA2, EIF4E and through its N-terminus with PAPBC1. Interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2. Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate EIF4E. Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA. Rapamycin can attenuate insulin stimulation mediated by FKBPs. Interacts with EIF4E3. Interacts with MIF4GD. Interacts with rotavirus A NSP3; in this interaction, NSP3 takes the place of PABPC1 thereby inducing shutoff of host protein synthesis

De Gregorio, E. et al. (1998) RNA 4, 828-836.
Ohlmann, T. et al. (1996) EMBO J. 15, 1371-1382.
Borman, A.M. and Kean, K.M. (1997) Virology 237, 129-136.
Gradi, A. et al. (1998) Mol Cell Biol 18, 334-42.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:100-1:300

Validation Images & Assay Conditions

Gene/Protein Information For GDF5 (Source: Uniprot.org, NCBI)

Gene Name

GDF5

Full Name

Growth/differentiation factor 5

Weight

55.411kDa

Superfamily

TGF-beta family

Alternative Names

BMP14; BMP-14; Cartilage-derived morphogenetic protein 1; cartilage-derived morphogenetic protein-1; CDMP-1; CDMP1LAP4; GDF5; GDF-5; growth differentiation factor 5; growth/differentiation factor 5; OS5; radotermin; SYNS2 GDF5 BDA1C, BMP-14, BMP14, CDMP1, DUPANS, LAP-4, LAP4, OS5, SYM1B, SYNS2 growth differentiation factor 5 growth/differentiation factor 5|LPS-associated protein 4|bone morphogenetic protein 14|cartilage-derived morphogenetic protein-1|lipopolysaccharide-associated protein 4|radotermin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GDF5, check out the GDF5 Infographic

GDF5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GDF5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for A01356-1

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-GDF-5 Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-GDF-5 Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

7 Customer Q&As for Anti-GDF-5 Antibody

Question

See attached the WB image, lot number and protocol we used for saliva-secreting gland using anti-GDF-5 antibody A01356-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-09-24

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-24

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for saliva-secreting gland using anti-GDF-5 antibody A01356-1. Let me know if you need anything else.

K. Patel

Verified customer

Asked: 2019-01-22

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-01-22

Question

Is this A01356-1 anti-GDF-5 antibody reactive to the isotypes of GDF5?

Verified Customer

Verified customer

Asked: 2019-01-18

Answer

The immunogen of A01356-1 anti-GDF-5 antibody is Synthesized peptide derived from the Internal region of human GDF-5. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-01-18

Question

My question regarding product A01356-1, anti-GDF-5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-09-14

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01356-1 anti-GDF-5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-09-14

Question

Will anti-GDF-5 antibody A01356-1 work for IHC with saliva-secreting gland?

E. Johnson

Verified customer

Asked: 2017-11-17

Answer

According to the expression profile of saliva-secreting gland, GDF5 is highly expressed in saliva-secreting gland. So, it is likely that anti-GDF-5 antibody A01356-1 will work for IHC with saliva-secreting gland.

Boster Scientific Support

Answered: 2017-11-17

Question

We are currently using anti-GDF-5 antibody A01356-1 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?

E. Baker

Verified customer

Asked: 2016-05-31

Answer

The anti-GDF-5 antibody (A01356-1) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-05-31

Question

We are interested in to test anti-GDF-5 antibody A01356-1 on mouse saliva-secreting gland for research purposes, then I may be interested in using anti-GDF-5 antibody A01356-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

S. Jackson

Verified customer

Asked: 2013-10-23

Answer

The products we sell, including anti-GDF-5 antibody A01356-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2013-10-23

Order DetailsPrice
A01356-1

100uL

$399

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
A01356-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.