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Product Info Summary
| SKU: | A02355 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-GDF11 Antibody (N-term)
SKU/Catalog Number
A02355
Size
80 µl
Description
Boster Bio Anti-GDF11 Antibody (N-term) (Catalog # A02355). Tested in IHC-P, WB application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-GDF11 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02355)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This GDF11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-61 amino acids from the N-terminal region of human GDF11.
Reactive Species
A02355 is reactive to GDF11 in Human, Mouse
Calculated molecular weight
45.1 kDa
Background of GDF11
GDF11 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice and Xenopus suggest that this protein is involved in mesodermal formation and neurogenesis during embryonic development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02355 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:10-1:50
Validation Images & Assay Conditions
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All lanes : Anti-hGDF11-R47 at 1:2000 dilution
Lane 1: human heart lysate
Lane 2: K562 whole cell lysate
Lane 3: mouse kidney lysate
Lane 4: 293 whole cell lysate
Lane 5: Hela whole cell lysate
Lane 5: mouse brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 45 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with GDF11 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with GDF11 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Specific Publications For Anti-GDF11 Antibody (N-term) (A02355)
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