Product Info Summary
SKU: | A02501 |
---|---|
Size: | 100ug |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-GGA-1 Antibody
SKU/Catalog Number
A02501
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-GGA-1 Antibody (Catalog # A02501). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-GGA-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02501)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 330-360 of Human GGA-1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02501 is reactive to GGA1 in Human
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
61762 MW
Background of GGA1
GGA1 (also known as ADP-ribosylation factor binding protein, Golgi-localized, gamma ear-containing, ARF-binding protein 1, Gamma-adaptin related protein 1) plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. This protein mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. GGA1 is a monomeric protein that interacts with NECAP1, GGA2 and GGA3 as well as RABEP1, RABGEF1 and type-I membrane proteins SORT1, SORL1, LRP3, M6PR/CD-MPR, IGF2R/CI-MPR and BACE1. GGA1 is a ubiquitously membrane-associated protein that is localized to TGN and endosomes. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02501 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000 - 1:50,000
IP: 1:100
WB: 1:500 - 1:3,000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of GGA1 expression in HEK293 cells over-expressing the recombinant protein (arrowhead). GGA1 at 100KD was detected using rabbit anti-GGA1 Antigen Affinity purified polyclonal antibody (Catalog # A02501) at 1:1350. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Protein Target Info & Infographic
Gene/Protein Information For GGA1 (Source: Uniprot.org, NCBI)
Gene Name
GGA1
Full Name
ADP-ribosylation factor-binding protein GGA1
Weight
61762 MW
Superfamily
GGA protein family
Alternative Names
GGA-1 rabbit, GGA 1,GGA1, ADP-ribosylation factor binding protein, Golgi-localized gamma ear-containing ARF-binding protein 1, Gamma-adaptin related protein 1 GGA1 golgi associated, gamma adaptin ear containing, ARF binding protein 1 ADP-ribosylation factor-binding protein GGA1|ADP-ribosylation factor binding protein 1|gamma-adaptin related protein 1|golgi-localized, gamma ear-containing, ARF-binding protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GGA1, check out the GGA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GGA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GGA-1 Antibody (A02501)
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Customer Q&As
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6 Customer Q&As for Anti-GGA-1 Antibody
Question
I was wanting to use your anti-GGA-1 antibody for WB for human heart on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human heart identification?
M. Krishna
Verified customer
Asked: 2019-12-27
Answer
It shows on the product datasheet, A02501 anti-GGA-1 antibody has been tested for IP, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human heart in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-27
Question
Does anti-GGA-1 antibody A02501 work on goat WB with brain?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
Our lab technicians have not validated anti-GGA-1 antibody A02501 on goat. You can run a BLAST between goat and the immunogen sequence of anti-GGA-1 antibody A02501 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-19
Question
Is this A02501 anti-GGA-1 antibody reactive to the isotypes of GGA1?
Verified Customer
Verified customer
Asked: 2018-12-07
Answer
The immunogen of A02501 anti-GGA-1 antibody is This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 336-352 of Human GGA-1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-12-07
Question
I see that the anti-GGA-1 antibody A02501 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-07-07
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-07-07
Question
We are currently using anti-GGA-1 antibody A02501 for human tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?
E. Gonzalez
Verified customer
Asked: 2016-07-07
Answer
The anti-GGA-1 antibody (A02501) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-07-07
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for heart using anti-GGA-1 antibody A02501. Let me know if you need anything else.
D. Yang
Verified customer
Asked: 2016-04-18
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-04-18