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- Table of Contents
CAP1 antibody (PB9923) performs well in WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Western blot analysis of CAP1 using anti-CAP1 antibody (PB9923).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: rat spleen tissue lysates,
Lane 4: mouse thymus tissue lysates,
Lane 5: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAP1 antigen affinity purified polyclonal antibody (PB9923) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CAP1 at approximately 55-60 kDa. The expected band size for CAP1 is at 52 kDa.
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: rat spleen tissue lysates,
Lane 4: mouse thymus tissue lysates,
Lane 5: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAP1 antigen affinity purified polyclonal antibody (PB9923) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CAP1 at approximately 55-60 kDa. The expected band size for CAP1 is at 52 kDa.
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Anti-CAP1 Antibody Picoband®
CAP1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | WB |
Tested Applications
CAP1 antibody was positively detected by WB in:
human Hela whole cell, rat thymus tissue, rat spleen tissue, mouse thymus tissue, mouse spleen tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
Human
Tested
Rat
Tested
Mouse
Tested
Monkey
Predicted
Rabbit
Predicted
Dog
Predicted
Product Information
Product Name
Anti-CAP1 Antibody Picoband®
View all CAP1 Antibodies
View all CAP1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CAP1 recombinant protein (Position: A2-R123). Human CAP1 shares 92.6% and 93.4% amino acid (aa) sequence identity with mouse and rat CAP1, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55-60 kDa
Calculated Molecular Weight
51.9 kDa
Background of CAP1
Adenylyl cyclase-associated protein 1 is an enzyme that in humans is encoded by the CAP1 gene. This gene is mapped to 1p34.2. The protein encoded by this gene is related to the S. cerevisiae CAP protein, which is involved in the cyclic AMP pathway. And the human protein is able to interact with other molecules of the same protein, as well as with CAP2 and actin. Alternatively spliced transcript variants have been identified.
Cite This Product
Anti-CAP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9923)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Rabbit
96%
High Homology
Rat
93%
High Homology
Mouse
93%
High Homology
Dog
93%
High Homology
Cow
89%
Moderate Homology
Sheep
89%
Moderate Homology
Zebrafish
50%
Low homology
Drosophila
38%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CAP1 antibody
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Product Promise
We promise Anti-CAP1 Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9923
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 31, 2026