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Product Info Summary
| SKU: | A12558 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-GTPase IMAP family member 2 GIMAP2 Antibody
SKU/Catalog Number
A12558
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-GTPase IMAP family member 2 GIMAP2 Antibody (Catalog # A12558). Tested in WB, IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GTPase IMAP family member 2 GIMAP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12558)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human GIMAP2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12558 is reactive to GIMAP2 in Human
Applications
A12558 is guaranteed for IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
38.017kDa
Background of GIMAP2
The heterodimer formed by GIMAP2 and GIMAP7 has GTPase activity. In contrast, GIMAP2 has no GTPase activity by itself.
The German cDNA consortium; Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Western blot analysis of extracts from HT-29 cells, using GIMAP2 antibody A12558.
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Immunofluorescence analysis of A549 cells, using GIMAP2 antibody A12558.
Protein Target Info & Infographic
Gene/Protein Information For GIMAP2 (Source: Uniprot.org, NCBI)
Gene Name
GIMAP2
Full Name
GTPase IMAP family member 2
Weight
38.017kDa
Superfamily
TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily
Alternative Names
DKFZp586D0824; GTPase, IMAP family member 2; HIMAP2; IMAP2GTPase IMAP family member 2; immunity associated protein 2; Immunity-associated protein 2; MGC24275 GIMAP2 HIMAP2, IAN12, IMAP2 GTPase, IMAP family member 2 GTPase IMAP family member 2|immune-associated nucleotide-binding protein 12|immunity associated protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GIMAP2, check out the GIMAP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GIMAP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GTPase IMAP family member 2 GIMAP2 Antibody (A12558)
Hello CJ!
No publications found for A12558
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Anti-GTPase IMAP family member 2 GIMAP2 Antibody
Question
See below the WB image, lot number and protocol we used for blood using anti-GIMAP2 antibody A12558. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-21
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-GIMAP2 antibody A12558. Let me know if you need anything else.
E. Dhar
Verified customer
Asked: 2019-12-10
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-10
Question
Would A12558 anti-GIMAP2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
As indicated on the product datasheet, A12558 anti-GIMAP2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-27
Question
I have a question about product A12558, anti-GIMAP2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A12558 anti-GIMAP2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-26
Question
Does anti-GIMAP2 antibody A12558 work on feline IF with uterus?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
Our lab technicians have not tested anti-GIMAP2 antibody A12558 on feline. You can run a BLAST between feline and the immunogen sequence of anti-GIMAP2 antibody A12558 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline uterus in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-18
Question
Is there a BSA free version of anti-GIMAP2 antibody A12558 available?
Verified Customer
Verified customer
Asked: 2017-12-06
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GIMAP2 antibody A12558 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-12-06
Question
I see that the anti-GIMAP2 antibody A12558 works with WB, what is the protocol used to produce the result images on the product page?
J. Lewis
Verified customer
Asked: 2017-02-08
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-02-08
