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Product Info Summary
| SKU: | A03282-2 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-Girdin CCDC88A Antibody
SKU/Catalog Number
A03282-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Girdin CCDC88A Antibody (Catalog # A03282-2). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Girdin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Girdin CCDC88A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03282-2)
Host
Rabbit
Contents
Girdin Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Girdin antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus human Girdin. The immunogen is located within the last 50 amino acids of Girdin.
Reactive Species
A03282-2 is reactive to CCDC88A in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
216.0 kDa
Background of CCDC88A
Girdin is a Galpha-interacting protein that can enhance the activation of the protein kinase Akt, remodel the actin cytoskeleton, and is thought to be involved in the regulation of cell migration and cancer metastasis. It has recently been shown that Girdin interacts with Disrupted-in-Schizophrenia 1 (DISC1), a susceptibility gene for major psychiatric disorders. DISC1 is thought to be involved in the migration, positioning and differentiation of dentate granule cells (DGCs) during development; depletion of Girdin or blocking the Girdin-DISC1 interaction results in defects in axonal sprouting in the CA3 region of the hippocampus and overextended migration of mispositioning of DGCs, suggesting the Girdin plays a role in postnatal neurogenesis in the dentate gyrus.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03282-2 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Girdin antibody can be used for detection of Girdin by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Girdin in rat brain tissue lysate with Girdin antibody at (A) 1 and (B) 2 μg/mL.
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Immunofluorescence of Girdin in human brain tissue with Girdin antibody at 20 μg/mL.
Specific Publications For Anti-Girdin CCDC88A Antibody (A03282-2)
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