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Product Info Summary
| SKU: | A03199-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-GJB3 Antibody
SKU/Catalog Number
A03199-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-GJB3 Antibody catalog # A03199-1. Tested in WB, IHC, ICC/IF, IP, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Cite This Product
Anti-GJB3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03199-1)
Host
Rabbit
Contents
500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
E.coli-derived human GJB3 recombinant protein (Position: 103-267).
Reactive Species
A03199-1 is reactive to GJB3 in Human, Mouse, Rat
Observed Molecular Weight
31 kDa
Calculated molecular weight
30.8 kDa
Background of GJB3
This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene can cause non-syndromic deafness or erythrokeratodermia variabilis, a skin disorder. Alternative splicing results in multiple transcript variants encoding the same protein.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03199-1 is guaranteed for ELISA, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Immunohistochemistry, 1:50-400
Immunocytochemistry/Immunofluorescence, 1:50-400
Immunoprecipitation, 1:250-300
ELISA, 1:100-1000
Positive Control
WB: human Hela whole cell, human PC-3 whole cell, human RT4 whole cell, rat stomach tissue, rat ovary tissue, mouse ovary tissue
Validation Images & Assay Conditions
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Western blot analysis of GJB3 using anti-GJB3 antibody (A03199-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: rat stomach tissue lysates,
Lane 5: rat ovary tissue lysates,
Lane 6: mouse ovary tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GJB3 antigen affinity purified polyclonal antibody (A03199-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GJB3 at approximately 31 kDa. The expected band size for GJB3 is at 31 kDa.
Specific Publications For Anti-GJB3 Antibody (A03199-1)
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