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Product Info Summary
| SKU: | A30492 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-GK3 GK3P Antibody
SKU/Catalog Number
A30492
Size
100ul
Form
Liquid
Description
Boster Bio Anti-GK3 GK3P Antibody (Catalog# A30492). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GK3 GK3P Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30492)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human GK3. Immunogen sequence location: 21-70
Reactive Species
A30492 is reactive to GK3P in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
61.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30492 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:40000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Western Blot analysis of various cells using GK1/3 Polyclonal Antibody
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Western blot analysis of lysates from 293 cells, using GK3 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-GK3 GK3P Antibody (A30492)
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Customer Q&As
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5 Customer Q&As for Anti-GK3 GK3P Antibody
Question
Does anti-GK3 antibody A30492 work for WB with brain?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
According to the expression profile of brain, GK3P is highly expressed in brain. So, it is likely that anti-GK3 antibody A30492 will work for WB with brain.
Boster Scientific Support
Answered: 2020-02-20
Question
We are currently using anti-GK3 antibody A30492 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
The anti-GK3 antibody (A30492) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-22
Question
Please see the WB image, lot number and protocol we used for brain using anti-GK3 antibody A30492. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-13
Question
I was wanting to use your anti-GK3 antibody for WB for human brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain identification?
M. Johnson
Verified customer
Asked: 2018-09-25
Answer
You can see on the product datasheet, A30492 anti-GK3 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-25
Question
Will anti-GK3 antibody A30492 work on primate WB with brain?
Verified Customer
Verified customer
Asked: 2017-08-09
Answer
Our lab technicians have not tested anti-GK3 antibody A30492 on primate. You can run a BLAST between primate and the immunogen sequence of anti-GK3 antibody A30492 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-09
