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Product Info Summary
| SKU: | A32403 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, IHC-P, WB |
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Product info
Product Name
Anti-GLD2 Antibody (N-term)
SKU/Catalog Number
A32403
Size
80 µl
Description
Boster Bio Anti-GLD2 Antibody (N-term) (Catalog # A32403). Tested in IF, IHC-P, WB application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-GLD2 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A32403)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This GLD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-87 amino acids from the N-terminal region of human GLD2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A32403 is reactive to TENT2 in Human, Mouse
Reconstitution
Calculated molecular weight
56028 Da
Background of Tent2
GLD2 is cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. GLD2 does not play a role in replication-dependent histone mRNA degradation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A32403 is guaranteed for IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:1000
IHC-P: 1:25
Validation Images & Assay Conditions
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Fluorescent image of MCF-7 cells stained with GLD2 Antibody (N-term). A32403 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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Western blot analysis of lysates from mouse brain, mouse cerebellum tissue lysate (from left to right), using GLD2 Antibody (N-term) (Cat. #A32403). A32403 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
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Western blot analysis of lysates from HepG2 cell line and mouse brain tissue lysate (from left to right), using GLD2 Antibody (N-term) (Cat. #A32403). A32403 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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GLD2 Antibody (N-term) (Cat. #A32403) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GLD2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Immunohistochemical analysis of paraffin-embedded H. cerebellum section using GLD2 Antibody (N-term) (Cat#A32403). A32403 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Protein Target Info & Infographic
Gene/Protein Information For TENT2 (Source: Uniprot.org, NCBI)
Gene Name
TENT2
Full Name
Poly(A) RNA polymerase GLD2
Weight
56028 Da
Superfamily
DNA polymerase type-B-like family
Alternative Names
Poly(A) RNA polymerase GLD2, hGLD-2, PAP-associated domain-containing protein 4, Terminal uridylyltransferase 2, TUTase 2, PAPD4, GLD2 Tent2|8030446C20Rik, AI649009, GLD-2, Gld2, Pa, Papd4|terminal nucleotidyltransferase 2|poly(A) RNA polymerase GLD2|PAP associated domain containing 4|PAP-associated domain-containing protein 4|cytoplasmic poly(A) polymerase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TENT2, check out the TENT2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TENT2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GLD2 Antibody (N-term) (A32403)
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