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- Table of Contents
SLC2A9 antibody (PB9891) performs well in Flow Cytometry, IF, IHC, WB and shows reactivity with Human, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Flow Cytometry analysis of U937 cells using anti-GLUT9 antibody (PB9891).
Overlay histogram showing U937 cells stained with PB9891 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GLUT9 Antibody (PB9891,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing U937 cells stained with PB9891 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GLUT9 Antibody (PB9891,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-GLUT9/SLC2A9 Antibody Picoband®
SLC2A9 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Rat | Rabbit | Flow Cytometry, IF, IHC, WB |
Tested Applications
SLC2A9 antibody was positively detected by WB in:
HepG2 whole cell, A549 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SLC2A9 antibody was positively detected by IHC in:
human renal cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
SLC2A9 antibody was positively detected by IF in:
human placenta tissue, human liver cancer tissue, rat intestinal tissue
SLC2A9 antibody was positively detected by FC in:
U937 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Immunofluorescence
2μg/ml
Human, Rat
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Product Information
Product Name
Anti-GLUT9/SLC2A9 Antibody Picoband®
View all SLC2A9 Antibodies
View all SLC2A9 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human GLUT9.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Calculated Molecular Weight
58.7 kDa
Background of SLC2A9
Solute carrier family 2, facilitated glucose transporter member 9, also known as SLC2A9, is a protein that in humans is encoded by the SLC2A9 gene. This gene encodes a member of the SLC2A facilitative glucose transporter family. Members of this family play a significant role in maintaining glucose homeostasis. This gene is mapped to 4p16.1. The encoded protein may play a role in the development and survival of chondrocytes in cartilage matrices. This gene transports urate and fructose. It may have a role in the urate reabsorption by proximal tubules. This gene also transports glucose at low rate.
Cite This Product
Anti-GLUT9/SLC2A9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9891)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
86%
Moderate Homology
Rat
86%
Moderate Homology
Dog
83%
Moderate Homology
Rabbit
78%
Moderate Homology
Cow
72%
Moderate Homology
Sheep
69%
Low homology
Monkey
64%
Low homology
Zebrafish
64%
Low homology
Drosophila
39%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for SLC2A9 antibody
Immunohistochemistry (IHC) protocol for SLC2A9 antibody
Immunofluorescence (IF) protocol for SLC2A9 antibody
Flow Cytometry (FC) protocol for SLC2A9 antibody
Publications
Application:
Species:
Targeting benign prostate hyperplasia treatments: AR/TGF-β/NOX4 inhibition by apocynin suppresses inflammation and proliferation
Published: 2023-04-14
Journal: Journal of Advanced Research
Application: WB
Impact Factor: 10.70
Species: Rat
PMID: 37061215
Review
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Product Promise
We promise Anti-GLUT9/SLC2A9 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
PB9891
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026