Anti-Glycogen Synthase 1 phospho S641 GYS1 Antibody

Product Name

Anti-Glycogen Synthase 1 phospho S641 GYS1 Antibody

View all Glycogen Synthase Antibodies

SKU/Catalog Number

P03512-3

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Glycogen Synthase 1 phospho S641 GYS1 Antibody (Catalog # P03512-3). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store Phospho GYS Antibody at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Glycogen Synthase 1 phospho S641 GYS1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P03512-3)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

Human Muscle Glycogen Synthase phospho peptide corresponding to a S641 region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P03512-3 is reactive to GYS1 in Human, Mouse

Applications

P03512-3 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

83.786kDa

Background of Glycogen Synthase

Anti-Glycogen synthase 1 pS641 is validated by IHC, Western Blot and ELISA. Human muscle glycogen synthase (GS) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GS is inactivated by the serine/threonine kinase called glycogen synthase kinase-3b that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GS at S641. Phosphorylation of GS at S641 has been associated with Antiphospholipid Antibody Syndrome.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:20,000 - 1:60,000
IHC: 1:1,000 - 1:5,000
WB: 1:1,000 - 1:10,000

Validation Images & Assay Conditions

Click image to see more details

Diagram of glycogen synthase as a component of insulin signal transduction pathways.

Click image to see more details

Affinity Purified Phospho-specific pS641 antibody to human muscle Glycogen Synthase (GS).Lane 1: mouse cardiac myocyte lysate mock treated. Lane 2: mouse cardiac myocyte lysate insulin treated at 10nM for 15'.Lane 3: mouse cardiac myocyte lysate insulin treated at 100nM for 15'.Lane 4: mouse cardiac myocyte lysate insulin treated at 1nM for 15'.Lane 5: mouse cardiac myocyte lysate mock treated. Lane 6: mouse cardiac myocyte lysate CLA treated at 4nM for 45'.Lane 7: mouse cardiac myocyte lysate CLA treated at 20nM for 45'.Lane 8: mouse cardiac myocyte lysate CLA treated at 100nM for 45'.Load: 12

Click image to see more details

Figure 1. IHC analysis of GYS1 using anti-GYS1 antibody (P03512-3).
GYS1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GYS1 Antibody (P03512-3) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Gene/Protein Information For GYS1 (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.4.1.11; glycogen [starch] synthase, muscle; glycogen synthase 1 (muscle); GYSGSY GYS1 GSY, GYS glycogen synthase 1 glycogen [starch] synthase, muscle|glycogen synthase 1 (muscle)

For more info on GYS1, check out the GYS1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GYS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the GYS1 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis