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GPC1 antibody (A03871-2) performs well in ELISA, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of Glypican 1/GPC1 using anti-Glypican 1/GPC1 antibody (A03871-2).
Glypican 1/GPC1 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Glypican 1/GPC1 Antibody (A03871-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Glypican 1/GPC1 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Glypican 1/GPC1 Antibody (A03871-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Glypican 1/GPC1 Antibody Picoband®
GPC1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IHC, WB |
Tested Applications
GPC1 antibody was positively detected by WB in:
human A431 whole cell, human U2OS whole cell, human RT4 whole cell, human U251 whole cell, rat heart tissue, rat brain tissue, mouse heart tissue, mouse brain tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
GPC1 antibody was positively detected by IHC in:
human pancreas cancer tissue, human lung cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
ELISA
0.1-0.5 μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Glypican 1/GPC1 Antibody Picoband®
View all GPC1 Antibodies
View all GPC1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human Glypican 1/GPC1 recombinant protein (Position: E72-N472). Human Glypican 1/GPC1shares 89.8% and 90% amino acid (aa) sequence identity with mouse and rat Glypican 1/GPC1, respectively.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
62 kDa
Calculated Molecular Weight
61.7 kDa
Background of GPC1
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.
Cite This Product
Anti-Glypican 1/GPC1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03871-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Rat
90%
High Homology
Mouse
90%
High Homology
Dog
89%
Moderate Homology
Cow
89%
Moderate Homology
Sheep
87%
Moderate Homology
Zebrafish
54%
Low homology
Drosophila
33%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for GPC1 antibody
Immunohistochemistry (IHC) protocol for GPC1 antibody
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Product Promise
We promise Anti-Glypican 1/GPC1 Antibody Picoband® performs excellently in relevant applications for ELISA, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A03871-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026