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Product Info Summary
| SKU: | PA1456 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-GM-CSF/CSF2 Antibody Picoband®
SKU/Catalog Number
PA1456
BA0748-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GM-CSF/CSF2 Antibody catalog # PA1456. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GM-CSF/CSF2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1456)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human GM-CSF.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1456 is reactive to CSF2 in Human
Observed Molecular Weight
35 kDa
Calculated molecular weight
16.3 kDa
Background of CSF2
GM-CSF, Granulocyte-macrophage colony-stimulating factor, is a protein secreted by macrophages, T cells, mast cells, endothelial cells, and fibroblasts. By fluorescence in situ hybridization, the GM-CSF gene is mapped to 5q31.1. GM-CSF is a cytokine that functions as a white blood cell growth factor. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. GM-CSF is an essential regulator of neutrophil function.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1456 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: JURKAT Whole Cell, HELA Whole Cell, HT1080Whole Cell
Validation Images & Assay Conditions
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Anti-GM-CSF antibody, PA1456, Western blotting
All lanes: Anti GM-CSF (PA1456) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: HT1080Whole Cell Lysate at 40ug
Predicted bind size: 16KD
Observed bind size: 35KD
Specific Publications For Anti-GM-CSF/CSF2 Antibody Picoband® (PA1456)
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Customer Q&As
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4 Customer Q&As for Anti-GM-CSF/CSF2 Antibody Picoband®
Question
We are currently using anti-GM-CSF/CSF2 antibody PA1456 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
The anti-GM-CSF/CSF2 antibody (PA1456) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-24
Question
My team were content with the WB result of your anti-GM-CSF/CSF2 antibody. However we have seen positive staining in upper lobe of left lung secreted using this antibody. Is that expected? Could you tell me where is CSF2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
From literature, upper lobe of left lung does express CSF2. Generally CSF2 expresses in secreted. Regarding which tissues have CSF2 expression, here are a few articles citing expression in various tissues:
Peripheral blood, Pubmed ID: 1737041
Boster Scientific Support
Answered: 2020-01-03
Question
We have seen staining in human peripheral blood. Any tips? Is anti-GM-CSF/CSF2 antibody supposed to stain peripheral blood positively?
Verified Customer
Verified customer
Asked: 2018-04-17
Answer
According to literature peripheral blood does express CSF2. According to Uniprot.org, CSF2 is expressed in upper lobe of left lung, peripheral blood, among other tissues. Regarding which tissues have CSF2 expression, here are a few articles citing expression in various tissues:
Peripheral blood, Pubmed ID: 1737041
Boster Scientific Support
Answered: 2018-04-17
Question
I am interested in using your anti-GM-CSF/CSF2 antibody for positive regulation of podosome assembly studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.
N. Jones
Verified customer
Asked: 2016-04-27
Answer
Thank you for your inquiry. This PA1456 anti-GM-CSF/CSF2 antibody is validated on jurkat whole cell lysate, hela whole cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-04-27
