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Product Info Summary
| SKU: | M01243 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-GNAS Antibody (N-Term)
SKU/Catalog Number
M01243
Size
50 µl
Description
Boster Bio Anti-GNAS Antibody (N-Term) (Catalog # M01243). Tested in WB application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-GNAS Antibody (N-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01243)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This GNAS antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 51-83 amino acids from human GNAS.
Reactive Species
M01243 is reactive to GNAS in Human, Mouse, Rat
Calculated molecular weight
45.7 kDa
Background of GNAS
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(s) protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli. Stimulates the Ras signaling pathway via RAPGEF2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01243 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:8000
Validation Images & Assay Conditions
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All lanes : Anti-GNAS Antibody (N-Term) at 1:8000 dilution
Lane 1: HepG2 whole cell lysate
Lane 2: Raji whole cell lysate
Lane 3: mouse brain lysate
Lane 4: rat brain lysate
Lane 5: human cerebellum lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 46 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-GNAS Antibody (N-Term) (M01243)
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