Anti-GNB1 Antibody Picoband™ (monoclonal, 11F9)

Product Name

Anti-GNB1 Antibody Picoband™ (monoclonal, 11F9)

View all GNB1 Antibodies

SKU/Catalog Number

M04650

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-GNB1 Antibody Picoband™ (monoclonal, 11F9) catalog # M04650. Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-GNB1 Antibody Picoband™ (monoclonal, 11F9) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04650)

Host

Mouse

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Monoclonal

Clone Number

11F9

Isotype

Mouse IgG2b

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human GNB1, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M04650 is reactive to GNB1 in Human, Mouse, Rat

Applications

M04650 is guaranteed for IF, ICC, WB Boster Guarantee

Observed Molecular Weight

37 kDa

Calculated molecular weight

37.377kDa

Background of GNB1

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 is a protein that in humans is encoded by the GNB1 gene. Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. This gene uses alternative polyadenylation signals.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of GNB1 using anti-GNB1 antibody (M04650).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat kidney tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-GNB1 antigen affinity purified monoclonal antibody (Catalog # M04650) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for GNB1 at approximately 37 kDa. The expected band size for GNB1 is at 37 kDa.

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Figure 2. IF analysis of GNB1 using anti-GNB1 antibody (M04650).
GNB1 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-GNB1 Antibody (M04650) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For GNB1 (Source: Uniprot.org, NCBI)

Alternative Names

beta subunit, signal-transducing proteins GS/GI; G protein, beta-1 subunit; guanine nucleotide binding protein (G protein), beta polypeptide 1; guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 1; guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1; Transducin beta chain 1 GNB1 MDS, MRD42 G protein subunit beta 1 guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1|beta subunit, signal-transducing proteins GS/GI|guanine nucleotide binding protein (G protein), beta polypeptide 1|testicular tissue protein Li 72|transducin beta chain 1

For more info on GNB1, check out the GNB1 Infographic

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In this infographic, you will see the following information for GNB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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