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Product Info Summary
| SKU: | M13925 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-GNG4 Antibody Picoband® (monoclonal, 7B6)
SKU/Catalog Number
M13925
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GNG4 Antibody Picoband® (monoclonal, 7B6) catalog # M13925. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-GNG4 Antibody Picoband® (monoclonal, 7B6) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M13925)
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
7B6
Isotype
Mouse IgG1
Immunogen
E.coli-derived human GNG4 recombinant protein (Position: M1-D52).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M13925 is reactive to GNG4 in Mouse, Rat
Observed Molecular Weight
12 kDa
Calculated molecular weight
8.4 kDa
Background of GNG4
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-4 is a protein that in humans is encoded by the GNG4 gene. This gene encodes the gamma subunit of the heterotrimeric G-proteins that are comprised of alpha, beta and gamma subunits. Upon activation by G protein-coupled receptors, the beta-gamma heterodimer dissociates from the alpha subunit to activate downstream signaling events. Alternate splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M13925 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Positive Control
WB: rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
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Western blot analysis of GNG4 using anti-GNG4 antibody (M13925).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-GNG4 antigen affinity purified monoclonal antibody (Catalog # M13925) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for GNG4 at approximately 12 kDa. The expected band size for GNG4 is at 12 kDa.
Specific Publications For Anti-GNG4 Antibody Picoband® (monoclonal, 7B6) (M13925)
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