Anti-GNPDA1 Antibody

Product Name

Anti-GNPDA1 Antibody

View all GNPDA1 Antibodies

SKU/Catalog Number

A10820-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-GNPDA1 Antibody (Catalog # A10820-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

GNPDA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-GNPDA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10820-1)

Host

Rabbit

Contents

GNPDA1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

GNPDA1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human GNPDA1. The immunogen is located within the last 50 amino acids of GNPDA1.

Cross-reactivity

GNPDA1 is predicted to not cross-react with GNPDA2.

Reactive Species

A10820-1 is reactive to GNPDA1 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

32.7 kDa

Background of GNPDA1

Glucosamine-6-phosphate deaminase (GNPDA) is an allosteric enzyme that catalyzes the reversible conversion of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium. GNPDA1 is one of two mammalian glucosamine-6-phosphate deaminases that are thought to have arisen though gene duplication, with the GNPDA2 protein closer in structure and activity to the E. coli enzyme. GNPDA1 possesses greater affinity for ammonium than either GNPDA2 or the E. coli enzyme suggesting that the forward reaction of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium is catalyzed at a slower rate than GNPDA2.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A10820-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

GNPDA1 antibody can be used for detection of GNPDA1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information GNPDA1

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