Anti-GnRH-Receptor / LH-RH Receptor Monoclonal Antibody

Product Name

Anti-GnRH-Receptor / LH-RH Receptor Monoclonal Antibody

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SKU/Catalog Number

M01442

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-GnRH-Receptor / LH-RH Receptor Monoclonal Antibody (Catalog # M01442). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Rat.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-GnRH-Receptor / LH-RH Receptor Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01442)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: F1G4

Isotype

IgG1, kappa

Immunogen

A synthetic peptide aa 1-29 (MANSASPEQNQHCSAINNSIPLMQGNLPY) from the N-terminal of human GnRH receptor.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M01442 is reactive to in Human, Rat

Applications

M01442 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

37731 MW

Background of

Recognizes an epitope on the extracellular domain of gonadotropin releasing hormone (GnRH) receptor or luteinizing hormone receptor (LHCGR). Lutropin (also designated luteinizing hormone) plays a role in spermatogenesis and ovulation by stimulating the testes and ovaries to produce steroids. Gonadotropin (also designated choriogonadotropin) production in the placenta maintains estrogen and progesterone levels during the first trimester of pregnancy. Ovaries and testes abundantly express luteinizing hormone/choriogonadotropin receptor. GnRH receptor contains seven hydrophobic transmembrane domains connected by hydrophilic extracellular and intracellular loops characteristic of G-protein coupled receptors. GnRH stimulates the gonadotrophs of the anterior pituitary to secrete luteinizing hormone (LH) as well as follicle-stimulating hormone (FSH). GnRH influences the protective effect of pregnancy and Gonadotropin against breast cancer. The expression of GnRH on breast carcinoma correlates in part to the degree of tumor differentiation. GnRH-positive breast tumors occur more frequently in tumors with greater cell differentiation in premenopausal women. GnRH is present in luteal and granulosa cells as well as in ovarian cell membrane preparations.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 8.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Ovarian Tumor stained with Anti-GnRH-Receptor Mouse Monoclonal Antibody (F1G4).

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Western Blot analysis of Human MDA-231 cell lysate using Anti-GnRH-Receptor Mouse Monoclonal Antibody (F1G4).

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Immunofluorescence analysis of Human MCF-7 cells labeling GnRH-R with Anti-GnRH-Receptor Mouse Monoclonal Antibody (F1G4) followed by Goat anti-mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

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