Anti-GOLGA3 Antibody Picoband™

Product Name

Anti-GOLGA3 Antibody Picoband™

View all GOLGA3 Antibodies

SKU/Catalog Number

A07522-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-GOLGA3 Antibody Picoband™ catalog # A07522-2. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-GOLGA3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07522-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human GOLGA3 recombinant protein (Position: Q176-K1436).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A07522-2 is reactive to GOLGA3 in Human

Applications

A07522-2 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

180 kDa

Calculated molecular weight

167.355kDa

Background of GOLGA3

Golgin subfamily A member 3 is a protein that in humans is encoded by the GOLGA3 gene. The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes a member of the golgin family of proteins which are localized to the Golgi. Its encoded protein has been postulated to play a role in nuclear transport and Golgi apparatus localization. Several alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of GOLGA3 using anti-GOLGA3 antibody (A07522-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GOLGA3 antigen affinity purified polyclonal antibody (Catalog # A07522-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GOLGA3 at approximately 180 kDa. The expected band size for GOLGA3 is at 167 kDa.

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Figure 2. IHC analysis of GOLGA3 using anti-GOLGA3 antibody (A07522-2).
GOLGA3 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GOLGA3 Antibody (A07522-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Figure 3. IHC analysis of GOLGA3 using anti-GOLGA3 antibody (A07522-2).
GOLGA3 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GOLGA3 Antibody (A07522-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Figure 4. IF analysis of GOLGA3 using anti-GOLGA3 antibody (A07522-2).
GOLGA3 was detected in an immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-GOLGA3 Antibody (A07522-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For GOLGA3 (Source: Uniprot.org, NCBI)

Alternative Names

GCP170Golgi peripheral membrane protein; golgi autoantigen, golgin subfamily a, 3; Golgi complex-associated protein of 170 kD; Golgi complex-associated protein of 170 kDa; Golgi membrane associated protein; golgin A3; Golgin subfamily A member 3; golgin-160; golgin-165; male enhanced antigen-2; MEA-2; SY2/SY10 protein GOLGA3 GCP170, MEA-2 golgin A3 golgin subfamily A member 3|Golgi membrane associated protein|Golgi peripheral membrane protein|SY2/SY10 protein|golgi autoantigen, golgin subfamily a, 3|golgi complex-associated protein of 170 kDa|golgin-160|golgin-165|male enhanced antigen-2

For more info on GOLGA3, check out the GOLGA3 Infographic

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In this infographic, you will see the following information for GOLGA3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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