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Product Info Summary
| SKU: | M13524-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Golgin 97/GOLGA1 Antibody Picoband® (monoclonal, 8E4H1)
SKU/Catalog Number
M13524-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Golgin 97/GOLGA1 Antibody Picoband® (monoclonal, 8E4H1) catalog # M13524-1. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Golgin 97/GOLGA1 Antibody Picoband® (monoclonal, 8E4H1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M13524-1)
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
8E4H1
Isotype
IgG1
Immunogen
E.coli-derived human Golgin 97/GOLGA1 recombinant protein (Position: M1-K752).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M13524-1 is reactive to GOLGA1 in Human
Observed Molecular Weight
97 kDa
Calculated molecular weight
88.2 kDa
Background of GOLGA1
Golgin subfamily A member 1 is a protein that in humans is encoded by the GOLGA1 gene. The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein is associated with Sjogren's syndrome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M13524-1 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
Positive Control
WB: human U-87MG whole cell, human Hela whole cell, human PC-3 whole cell, human HepG2 whole cell
ICC/IF: Hela cell
FCM: A549 cell
Validation Images & Assay Conditions
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Western blot analysis of Golgin 97/GOLGA1 using anti-Golgin 97/GOLGA1 antibody (M13524-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Golgin 97/GOLGA1 antigen affinity purified monoclonal antibody (Catalog # M13524-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Golgin 97/GOLGA1 at approximately 97 kDa. The expected band size for Golgin 97/GOLGA1 is at 88 kDa.
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IF analysis of Golgin 97/GOLGA1 using anti-Golgin 97/GOLGA1 antibody (M13524-1).
Golgin 97/GOLGA1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-Golgin 97/GOLGA1 Antibody (M13524-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Flow Cytometry analysis of A549 cells using anti-Golgin 97/GOLGA1 antibody (M13524-1).
Overlay histogram showing A549 cells stained with M13524-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Golgin 97/GOLGA1 Antibody (M13524-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-Golgin 97/GOLGA1 Antibody Picoband® (monoclonal, 8E4H1) (M13524-1)
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