Anti-GPAT1 GPAM Antibody

Product Name

Anti-GPAT1 GPAM Antibody

View all Gpam Antibodies

SKU/Catalog Number

A05432

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-GPAT1 GPAM Antibody (Catalog # A05432). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

GPAT1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-GPAT1 GPAM Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05432)

Host

Rabbit

Contents

GPAT1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

GPAT1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of the human GPAT1. The immunogen is located within amino acids 730 - 780 of GPAT1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least four isoforms of GPAT1 are known to exist; this antibody will detect only the two largest isoforms.

Reactive Species

A05432 is reactive to Gpam in Human, Mouse, Rat

Applications

A05432 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

93.705kDa

Background of Gpam

Glycerol-3-phosphate acyltransferase 1 (GPAT1), one of four known GPAT isoforms, is located on the mitochondrial outer membrane, allowing reciprocal regulation with carnitine palmitoyltransferase-1. It is thought to be critical for the development of hepatic steatosis; steatosis triggered by GPAT1 overexpression leads to hepatic and possibly peripheral insulin resistance. GPAT1 is transcriptionally upregulated by insulin and sterol regulatory element binding protein (SREBP-1) and downregulated by AMP-activated protein kinase. Mice deficient in GPAT1 exhibit decreased triacylglycerol (TAG) in cardiomyocytes even in high-fat diets, suggesting that GPAT1 contributes significantly to TAG accumulation in heart tissue during lipogenic or high fat diets.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

GPAT1 antibody can be used for detection of GPAT1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of GPAT1 in rat brain tissue lysate with GPAT1 antibody at (A) 1 and (B) 2 μg/mL.

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Immunohistochemistry of GPAT1 in rat brain with GPAT1 antibody at 2.5 μg/mL.

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Immunofluorescence of GPAT1 in Rat Brain tissue with GPAT1 antibody at 20 μg/mL.

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Immunohistochemistry of GPAT1 in mouse brain tissue with GPAT1 antibody at 5 μg/ml.

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Immunofluorescence of GPAT1 in mouse brain tissue with GPAT1 antibody at 20 μg/mL.

Green: GPAT1 Antibody (A05432)
Blue: DAPI staining

Gene/Protein Information For Gpam (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.3.1; EC 2.3.1.15; glycerol-3-phosphate acyltransferase 1, mitochondrial; glycerol-3-phosphate acyltransferase, mitochondrial; GPAT; GPAT-1; GPAT1mitochondrial; MGC26846 Gpam|GPA, GPAT, GPAT-1, GPAT1, P90|glycerol-3-phosphate acyltransferase, mitochondrial|glycerol-3-phosphate acyltransferase 1, mitochondrial

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In this infographic, you will see the following information for Gpam: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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