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Product Info Summary
| SKU: | A08247-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-GPCR GPR7/NPBWR1 Antibody Picoband®
SKU/Catalog Number
A08247-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GPCR GPR7/NPBWR1 Antibody Picoband® catalog # A08247-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GPCR GPR7/NPBWR1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08247-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human GPCR GPR7/NPBWR1 recombinant protein (Position: V58-A328).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A08247-1 is reactive to NPBWR1 in Human, Mouse, Rat
Observed Molecular Weight
43 kDa
Calculated molecular weight
36.1 kDa
Background of NPBWR1
Neuropeptides B/W receptor 1, also known as NPBW1 and GPR7, is a human protein encoded by the NPBWR1 gene. In rodent models, NPBWR1 is over-expressed in Schwann cells associated with neuropathic pain, suggesting it inhibits inflammatory pain responses. Mice without NPBW1 exhibited a stronger hostile reaction to intruders, suggesting NPBW1 has a role in stress responses. Early studies indicated that NPB and NPW had a complex effect on appetite, but generally led to anorexia. Similarly, male rats lacking NPBWR1 exhibited hyperphagiaand adult-onset obesity, though why female rats are unaffected is unknown. Researchers speculated that activating these pathways might decrease obesity, and synthesized a small-molecule ligand that is capable of stimulating both receptors at low concentrations.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08247-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human placenta tissues, human U-937 whole cell, rat heart tissue, mouse heart tissue,
Validation Images & Assay Conditions
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Western blot analysis of NPBWR1 using anti-NPBWR1 antibody (A08247-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissues lysates
Lane 2: human U-937 whole cell lysates
Lane 3: rat heart tissue lysates
Lane 4: mouse heart tissue lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NPBWR1 antigen affinity purified polyclonal antibody (Catalog # A08247-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NPBWR1 at approximately 43KD. The expected band size for NPBWR1 is at 36KD.
Specific Publications For Anti-GPCR GPR7/NPBWR1 Antibody Picoband® (A08247-1)
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4 Customer Q&As for Anti-GPCR GPR7/NPBWR1 Antibody Picoband®
Question
Since A08247-1 sample contain cryoprotectants such as DMSO, would it freeze at -20°C?
Verified customer
Asked: 2022-04-11
Answer
The suggested storage condition for Anti-GPCR GPR7/NPBWR1 Antibody Picoband™ (A08247-1) sample, it should be stored at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months.
Boster Scientific Support
Answered: 2022-04-12
Question
We are currently using anti-GPCR GPR7/NPBWR1 antibody A08247-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
The anti-GPCR GPR7/NPBWR1 antibody (A08247-1) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-10
Question
Can you help my question with product A08247-1, anti-GPCR GPR7/NPBWR1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A08247-1 anti-GPCR GPR7/NPBWR1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-04
Question
Is a blocking peptide available for product anti-GPCR GPR7/NPBWR1 antibody (A08247-1)?
Verified Customer
Verified customer
Asked: 2018-02-14
Answer
We do provide the blocking peptide for product anti-GPCR GPR7/NPBWR1 antibody (A08247-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-02-14
