Click image to see more details
Product Info Summary
| SKU: | A09371 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-GPR100 RXFP4 Antibody
SKU/Catalog Number
A09371
Size
100μl
Form
Liquid
Description
Boster Bio Anti-GPR100 RXFP4 Antibody catalog # A09371. Tested in WB, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GPR100 RXFP4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09371)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human GPR100. AA range:321-370
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A09371 is reactive to RXFP4 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
41.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09371 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
Click image to see more details
Western Blot analysis of A549 cells using GPR100 Polyclonal Antibody
Click image to see more details
Western blot analysis of lysates from A549 cells, using GPR100 Antibody. The lane on the right is blocked with the synthesized peptide.
Click image to see more details
Immunofluorescence analysis of A549 cells, using GPR100 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-GPR100 RXFP4 Antibody (A09371)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-GPR100 RXFP4 Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-GPR100 RXFP4 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
4 Customer Q&As for Anti-GPR100 RXFP4 Antibody
Question
Would anti-GPR100 antibody A09371 work on canine WB with brain?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
Our lab technicians have not tested anti-GPR100 antibody A09371 on canine. You can run a BLAST between canine and the immunogen sequence of anti-GPR100 antibody A09371 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-11
Question
We are currently using anti-GPR100 antibody A09371 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
The anti-GPR100 antibody (A09371) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-08
Question
Does A09371 anti-GPR100 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Evans
Verified customer
Asked: 2015-08-18
Answer
It shows on the product datasheet, A09371 anti-GPR100 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-08-18
Question
I have attached the WB image, lot number and protocol we used for pancreatic carcinoma using anti-GPR100 antibody A09371. Please let me know if you require anything else.
Z. Singh
Verified customer
Asked: 2015-05-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-05-28
