Click image to see more details
Product Info Summary
| SKU: | A10226 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Monkey, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-GPR101 Antibody
SKU/Catalog Number
A10226
Size
100μl
Form
Liquid
Description
Boster Bio Anti-GPR101 Antibody catalog # A10226. Tested in WB, IF, ELISA applications. This antibody reacts with Human, Mouse, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GPR101 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10226)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human GPR101. AA range:451-500
Reactive Species
A10226 is reactive to GPR101 in Human, Monkey, Mouse
Calculated molecular weight
56.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10226 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:5000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using GPR101 Polyclonal Antibody diluted at 1:500
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Western blot analysis of lysates from COS7 cells, using GPR101 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of HeLa cells, using GPR101 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-GPR101 Antibody (A10226)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-GPR101 Antibody
Question
Please see the WB image, lot number and protocol we used for nucleus accumbens using anti-GPR101 antibody A10226. Please let me know if you require anything else.
C. Zhang
Verified customer
Asked: 2020-05-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-05-05
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for nucleus accumbens using anti-GPR101 antibody A10226. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-31
Question
Is there a BSA free version of anti-GPR101 antibody A10226 available?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GPR101 antibody A10226 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-17
Question
I was wanting to use your anti-GPR101 antibody for WB for human nucleus accumbens on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human nucleus accumbens identification?
C. Li
Verified customer
Asked: 2018-06-05
Answer
You can see on the product datasheet, A10226 anti-GPR101 antibody has been validated for WB on human, monkey, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human nucleus accumbens in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-05
Question
I was wanting to use to test anti-GPR101 antibody A10226 on human nucleus accumbens for research purposes, then I may be interested in using anti-GPR101 antibody A10226 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-04-20
Answer
The products we sell, including anti-GPR101 antibody A10226, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-04-20
Question
We are currently using anti-GPR101 antibody A10226 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, monkey, mouse. Is it likely that the antibody can work on horse tissues as well?
B. Rodriguez
Verified customer
Asked: 2017-07-25
Answer
The anti-GPR101 antibody (A10226) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-25
