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Product Info Summary
| SKU: | A30799 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-GPR114 Antibody
SKU/Catalog Number
A30799
Size
100ul
Form
Liquid
Description
Boster Bio Anti-GPR114 Antibody (Catalog# A30799). Tested in WB, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GPR114 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30799)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human GPR114. Immunogen sequence location: 91 - 140
Reactive Species
A30799 is reactive to ADGRG5 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
59.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30799 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
ICC/IF 1:200 - 1:1000.
ELISA: 1:5000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western Blot analysis of COLO205 cells using GPR114 Polyclonal Antibody
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Western blot analysis of lysates from COLO205 cells, using GPR114 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from 293 cells using GPR114 antibody.
Specific Publications For Anti-GPR114 Antibody (A30799)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-GPR114 Antibody
Question
I need a help with my WB results. What information do you need to help me?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
WB image, lot number and protocol we used for thymus trachea using anti-GPR114 antibody A30799.
Boster Scientific Support
Answered: 2020-05-08
Question
Is a blocking peptide available for product anti-GPR114 antibody (A30799)?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
We do provide the blocking peptide for product anti-GPR114 antibody (A30799). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-01-22
Question
We are currently using anti-GPR114 antibody A30799 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
P. Miller
Verified customer
Asked: 2018-04-09
Answer
The anti-GPR114 antibody (A30799) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-04-09
Question
Is this A30799 anti-GPR114 antibody reactive to the isotypes of ADGRG5?
S. Moore
Verified customer
Asked: 2013-02-26
Answer
The immunogen of A30799 anti-GPR114 antibody is A peptide derived from human GPR114. Immunogen sequence location: 91 - 140. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-02-26
