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Product Info Summary
| SKU: | A14764 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-GPR115 ADGRF4 Antibody
SKU/Catalog Number
A14764
Size
100μl
Form
Liquid
Description
Boster Bio Anti-GPR115 ADGRF4 Antibody catalog # A14764. Tested in WB, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GPR115 ADGRF4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14764)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human GPR115. AA range:641-690
Reactive Species
A14764 is reactive to ADGRF4 in Human, Mouse
Calculated molecular weight
77.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14764 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot analysis of RAW264.7 cells using GPR115 Polyclonal Antibody
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Western blot analysis of lysates from RAW264.7 cells, using GPR115 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of LOVO cells, using GPR115 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-GPR115 ADGRF4 Antibody (A14764)
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Customer Q&As
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4 Customer Q&As for Anti-GPR115 ADGRF4 Antibody
Question
Will anti-GPR115 antibody A14764 work on horse WB with tongue?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
Our lab technicians have not tested anti-GPR115 antibody A14764 on horse. You can run a BLAST between horse and the immunogen sequence of anti-GPR115 antibody A14764 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse tongue in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-03
Question
I have a question about product A14764, anti-GPR115 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A14764 anti-GPR115 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-27
Question
Is this A14764 anti-GPR115 antibody reactive to the isotypes of ADGRF4?
G. Banerjee
Verified customer
Asked: 2019-02-28
Answer
The immunogen of A14764 anti-GPR115 antibody is Synthesized peptide derived from the C-terminal region of human GPR115. at AA rangle: 620-700. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-02-28
Question
Is a blocking peptide available for product anti-GPR115 antibody (A14764)?
Verified Customer
Verified customer
Asked: 2018-12-19
Answer
We do provide the blocking peptide for product anti-GPR115 antibody (A14764). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-12-19
