Product Info Summary
SKU: | A04709 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-GPR120 FFAR4 Antibody
View all FFAR4/GPR120 Antibodies
SKU/Catalog Number
A04709
Size
100μl
Form
Liquid
Description
Boster Bio Anti-GPR120 FFAR4 Antibody catalog # A04709. Tested in ELISA, IF, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GPR120 FFAR4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04709)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human GPR120. at AA range: 190-270
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04709 is reactive to Ffar4 in Human, Mouse, Rat
Applications
A04709 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
40.813kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of HEPG2-UV cells using GPR120 Polyclonal Antibody diluted at 1:500
Click image to see more details
Western blot analysis of various cells using GPR120 Polyclonal Antibody diluted at 1:500
Protein Target Info & Infographic
Gene/Protein Information For Ffar4 (Source: Uniprot.org, NCBI)
Gene Name
Ffar4
Full Name
Free fatty acid receptor 4
Weight
40.813kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
FFAR4; G protein-coupled receptor 120; G protein-coupled receptor 129; G protein-coupled receptor PGR4; GPR120; GPR129; G-protein coupled receptor 120; G-protein coupled receptor 129; G-protein coupled receptor GT01; G-protein coupled receptor PGR4; G-protein-coupled receptor GT01; GT01; MGC119984; O3FAR1; omega-3 fatty acid receptor 1; PGR4; PGR4DKFZp686F0824 Ffar4|AI552415, Ffa, Ffa4, GT01, Gpr1, Gpr120, Gpr129, KPG_013, O3f, O3far1, Pg, Pgr4|free fatty acid receptor 4|free fatty acid receptor 4|G-protein coupled receptor 120|G-protein-coupled receptor GT01|omega-3 fatty acid receptor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ffar4, check out the Ffar4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ffar4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GPR120 FFAR4 Antibody (A04709)
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No publications found for A04709
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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12 Customer Q&As for Anti-GPR120 FFAR4 Antibody
Question
Does anti-GPR120 antibody A04709 work for WB with adenohypophysis?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
According to the expression profile of adenohypophysis, FFAR4 is highly expressed in adenohypophysis. So, it is likely that anti-GPR120 antibody A04709 will work for WB with adenohypophysis.
Boster Scientific Support
Answered: 2019-11-08
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adenohypophysis using anti-GPR120 antibody A04709. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-02
Question
I was wanting to use your anti-GPR120 antibody for WB for mouse adenohypophysis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse adenohypophysis identification?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
You can see on the product datasheet, A04709 anti-GPR120 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse adenohypophysis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-31
Question
Is this A04709 anti-GPR120 antibody reactive to the isotypes of FFAR4?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
The immunogen of A04709 anti-GPR120 antibody is Synthesized peptide derived from the Internal region of human GPR120. at AA rangle: 190-270. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-24
Question
Do you have a BSA free version of anti-GPR120 antibody A04709 available?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GPR120 antibody A04709 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-20
Question
Does A04709 anti-GPR120 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
It shows on the product datasheet, A04709 anti-GPR120 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-16
Question
Will anti-GPR120 antibody A04709 work on monkey WB with adenohypophysis?
M. Krishna
Verified customer
Asked: 2018-10-30
Answer
Our lab technicians have not validated anti-GPR120 antibody A04709 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-GPR120 antibody A04709 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey adenohypophysis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-30
Question
I see that the anti-GPR120 antibody A04709 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-03-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-03-16
Question
Here is the WB image, lot number and protocol we used for adenohypophysis using anti-GPR120 antibody A04709. Please let me know if you require anything else.
S. Evans
Verified customer
Asked: 2018-02-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-09
Question
I have a question about product A04709, anti-GPR120 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Thomas
Verified customer
Asked: 2017-09-25
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04709 anti-GPR120 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-09-25
Question
Is a blocking peptide available for product anti-GPR120 antibody (A04709)?
J. Wu
Verified customer
Asked: 2014-06-06
Answer
We do provide the blocking peptide for product anti-GPR120 antibody (A04709). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-06-06
Question
I am interested in to test anti-GPR120 antibody A04709 on mouse adenohypophysis for research purposes, then I may be interested in using anti-GPR120 antibody A04709 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
T. Walker
Verified customer
Asked: 2013-03-25
Answer
The products we sell, including anti-GPR120 antibody A04709, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-03-25