Anti-GPR132 Antibody

Product Name

Anti-GPR132 Antibody

View all G2A/GPR132 Antibodies

SKU/Catalog Number

A07182-2

Size

100ul

Form

Liquid

Description

Boster Bio Anti-GPR132 Antibody catalog # A07182-2. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GPR132 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07182-2)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human GPR132

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A07182-2 is reactive to GPR132 in Human

Applications

A07182-2 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

42.499kDa

Background of G2A/GPR132

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Myeloid leukemia factor-1 (MLF1) Interacting Protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a novel polo-like kinase 1 (Plk1) substrate. Plk1 phosphorylation of MLF1IP induces ubiquitination and degradation of MLF1IP prior to the metaphase/anaphase transition. Several Plk1-dependent phosphorylation sites have been identified on MLF1IP by mass spectrometry. Mutations of these sites stabilize MLF1IP and inhibit mitotic progression. Subsequent in vitro and in vivo MLF1IP phosphorylation and stability assays have revealed that phosphorylation of Thr78 is critical for triggering Plk1-dependent MLF1IP degradation. Expression of a non-degradable Thr78Ala mutant was sufficient to induce a mitotic block. Timely phosphorylation of MLF1IP on Thr78 by Plk1 is critical for eliminating the MLF1IP-imposed mitotic block prior to anaphase onset. MLF1IP is speculated to be a novel tumor suppressor, whose function is required for proper sister-chromatid separation. Loss of MLF1IP function may result in improper segregation of chromosomes and genomic instability, thus promoting tumorigenesis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

Gene/Protein Information For GPR132 (Source: Uniprot.org, NCBI)

Alternative Names

G protein-coupled receptor 132; G2 accumulation protein; G2A; G2AG protein-coupled receptor G2A; GPR132; MGC99642; probable G-protein coupled receptor 132 GPR132 G2A G protein-coupled receptor 132 probable G-protein coupled receptor 132|G2 accumulation protein

For more info on GPR132, check out the GPR132 Infographic

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In this infographic, you will see the following information for GPR132: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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