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Product Info Summary
| SKU: | A15465 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-GPR146 Antibody
SKU/Catalog Number
A15465
Size
100μl
Form
Liquid
Description
Boster Bio Anti-GPR146 Antibody catalog # A15465. Tested in WB, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GPR146 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15465)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human GPR146. AA range:191-240
Reactive Species
A15465 is reactive to GPR146 in Human, Mouse
Calculated molecular weight
36.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15465 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:5000
Validation Images & Assay Conditions
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Western Blot analysis of LOVO cells using GPR146 Polyclonal Antibody
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Western blot analysis of lysates from LOVO cells, using GPR146 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-GPR146 Antibody (A15465)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-GPR146 Antibody
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-GPR146 antibody A15465. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-16
Question
Would anti-GPR146 antibody A15465 work on pig WB with kidney?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
Our lab technicians have not tested anti-GPR146 antibody A15465 on pig. You can run a BLAST between pig and the immunogen sequence of anti-GPR146 antibody A15465 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig kidney in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-31
Question
See attached the WB image, lot number and protocol we used for kidney using anti-GPR146 antibody A15465. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-29
Question
Is this A15465 anti-GPR146 antibody reactive to the isotypes of GPR146?
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
The immunogen of A15465 anti-GPR146 antibody is Synthesized peptide derived from the Internal region of human GPR146. at AA rangle: 160-240. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-15
Question
Does anti-GPR146 antibody A15465 work for WB with kidney?
E. Collins
Verified customer
Asked: 2017-05-17
Answer
According to the expression profile of kidney, GPR146 is highly expressed in kidney. So, it is likely that anti-GPR146 antibody A15465 will work for WB with kidney.
Boster Scientific Support
Answered: 2017-05-17
Question
I have a question about product A15465, anti-GPR146 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Thomas
Verified customer
Asked: 2013-02-27
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A15465 anti-GPR146 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-02-27
