Anti-GPR149 Antibody

Gpr149 antibody

Boster Bio Anti-GPR149 Antibody (Catalog# A30812). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Rat, Mouse.

Product Info Summary

SKU: A30812
Size: 100ul
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IF, IHC-P, ICC, WB

Product Name

Anti-GPR149 Antibody

View all Gpr149 Antibodies

SKU/Catalog Number

A30812

Size

100ul

Form

Liquid

Description

Boster Bio Anti-GPR149 Antibody (Catalog# A30812). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Rat, Mouse.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GPR149 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30812)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A peptide derived from human GPR149. Immunogen sequence location: 451 - 500

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A30812 is reactive to Gpr149 in Human, Mouse, Rat

Applications

A30812 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

80.524kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:5000
Not yet tested in other applications.

Validation Images & Assay Conditions

Gene/Protein Information For Gpr149 (Source: Uniprot.org, NCBI)

Gene Name

Gpr149

Full Name

Probable G-protein coupled receptor 149

Weight

80.524kDa

Superfamily

G-protein coupled receptor 1 family

Alternative Names

G protein-coupled receptor 149; G protein-coupled receptor PGR10; GPR149; G-protein coupled receptor PGR10; IEDA; PGR10; PGR10IEDA; probable G-protein coupled receptor 149 Gpr149|Ieda|G protein-coupled receptor 149|probable G-protein coupled receptor 149|induced early in differentiating astrocytes gene protein|orphan seven transmembrane receptor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Gpr149, check out the Gpr149 Infographic

Gpr149 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Gpr149: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-GPR149 Antibody

Question

We are currently using anti-GPR149 antibody A30812 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-25

Answer

The anti-GPR149 antibody (A30812) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-25

Question

Will A30812 anti-GPR149 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-12-26

Answer

It shows on the product datasheet, A30812 anti-GPR149 antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-12-26

Question

Is there a BSA free version of anti-GPR149 antibody A30812 available?

Verified Customer

Verified customer

Asked: 2019-12-25

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GPR149 antibody A30812 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-12-25

Question

Is this A30812 anti-GPR149 antibody reactive to the isotypes of GPR149?

S. Zhao

Verified customer

Asked: 2019-11-13

Answer

The immunogen of A30812 anti-GPR149 antibody is A peptide derived from human GPR149. Immunogen sequence location: 451 - 500. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-11-13

Question

I have a question about product A30812, anti-GPR149 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

K. Gonzalez

Verified customer

Asked: 2015-09-21

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30812 anti-GPR149 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2015-09-21

Order DetailsPrice
A30812

100 μL

$335

Free Secondary Antibody

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A30812
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$335.00

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