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Product Info Summary
| SKU: | A30818 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-G-protein coupled receptor 157 GPR157 Antibody
SKU/Catalog Number
A30818
Size
100ul
Form
Liquid
Description
Boster Bio Anti-G-protein coupled receptor 157 GPR157 Antibody (Catalog# A30818). Tested in WB, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-G-protein coupled receptor 157 GPR157 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30818)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human GPR157. Immunogen sequence location: 81 - 130
Reactive Species
A30818 is reactive to GPR157 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
36.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30818 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
ICC/IF 1:200 - 1:1000.
ELISA: 1:5000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunofluorescence analysis of HUVEC cells, using GPR157 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from NIH/3T3 cells, using GPR157 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from RAW264.7cells using GPR157 antibody.
Specific Publications For Anti-G-protein coupled receptor 157 GPR157 Antibody (A30818)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-G-protein coupled receptor 157 GPR157 Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta prostate using anti-GPR157 antibody A30818. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-30
Question
We are currently using anti-GPR157 antibody A30818 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
The anti-GPR157 antibody (A30818) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-16
Question
Can you help my question with product A30818, anti-GPR157 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-30
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30818 anti-GPR157 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-30
Question
Does anti-GPR157 antibody A30818 work on primate IF with placenta prostate?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
Our lab technicians have not tested anti-GPR157 antibody A30818 on primate. You can run a BLAST between primate and the immunogen sequence of anti-GPR157 antibody A30818 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate placenta prostate in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-25
Question
Is a blocking peptide available for product anti-GPR157 antibody (A30818)?
Verified Customer
Verified customer
Asked: 2018-02-07
Answer
We do provide the blocking peptide for product anti-GPR157 antibody (A30818). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-02-07
Question
Does anti-GPR157 antibody A30818 work for IF with placenta prostate?
Verified Customer
Verified customer
Asked: 2017-11-28
Answer
According to the expression profile of placenta prostate, GPR157 is highly expressed in placenta prostate. So, it is likely that anti-GPR157 antibody A30818 will work for IF with placenta prostate.
Boster Scientific Support
Answered: 2017-11-28
