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Product Info Summary
| SKU: | A15335 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-GPR176 Antibody
SKU/Catalog Number
A15335
Size
100μl
Form
Liquid
Description
Boster Bio Anti-GPR176 Antibody catalog # A15335. Tested in WB, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GPR176 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15335)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human GPR176. AA range:466-515
Reactive Species
A15335 is reactive to GPR176 in Human, Mouse, Rat
Calculated molecular weight
57.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15335 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using GPR176 Polyclonal Antibody
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Western blot analysis of lysates from COLO205 cells, using GPR176 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of MCF7 cells, using GPR176 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-GPR176 Antibody (A15335)
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-GPR176 Antibody
Question
We are currently using anti-GPR176 antibody A15335 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
The anti-GPR176 antibody (A15335) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-12
Question
I was wanting to use your anti-GPR176 antibody for WB for human fetal brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human fetal brain identification?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
As indicated on the product datasheet, A15335 anti-GPR176 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human fetal brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-14
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal brain using anti-GPR176 antibody A15335. Let me know if you need anything else.
G. Johnson
Verified customer
Asked: 2013-04-29
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-04-29
