Click image to see more details
Product Info Summary
| SKU: | A30824 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-GPR180 Antibody
SKU/Catalog Number
A30824
Size
100ul
Form
Liquid
Description
Boster Bio Anti-GPR180 Antibody (Catalog# A30824). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GPR180 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30824)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human GPR180. Immunogen sequence location: 241 - 290
Reactive Species
A30824 is reactive to GPR180 in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
49.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30824 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:10000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using GPR180 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of A549 cells, using GPR180 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-GPR180 Antibody (A30824)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-GPR180 Antibody
Question
Is this A30824 anti-GPR180 antibody reactive to the isotypes of GPR180?
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
The immunogen of A30824 anti-GPR180 antibody is A peptide derived from human GPR180. Immunogen sequence location: 241 - 290. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-14
Question
We are currently using anti-GPR180 antibody A30824 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
The anti-GPR180 antibody (A30824) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-03
Question
Would A30824 anti-GPR180 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
It shows on the product datasheet, A30824 anti-GPR180 antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-31
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for buccal mucosa cell using anti-GPR180 antibody A30824. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-05
Question
I see that the anti-GPR180 antibody A30824 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-04-12
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-04-12
