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Product Info Summary
| SKU: | A14205 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-GPR20 Antibody
SKU/Catalog Number
A14205
Size
100ul
Form
Liquid
Description
Boster Bio Anti-GPR20 Antibody (Catalog# A14205). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GPR20 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14205)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human GPR20. Immunogen sequence location: 291 - 340
Reactive Species
A14205 is reactive to GPR20 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
38.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14205 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:5000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using GPR20 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from LOVO cells, using GPR20 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-GPR20 Antibody (A14205)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-GPR20 Antibody
Question
See below the WB image, lot number and protocol we used for lung placenta using anti-GPR20 antibody A14205. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-11
Question
Would anti-GPR20 antibody A14205 work on bovine IHC with lung placenta?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
Our lab technicians have not tested anti-GPR20 antibody A14205 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-GPR20 antibody A14205 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine lung placenta in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-05
Question
I see that the anti-GPR20 antibody A14205 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-08-20
Question
I was wanting to use to test anti-GPR20 antibody A14205 on human lung placenta for research purposes, then I may be interested in using anti-GPR20 antibody A14205 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
The products we sell, including anti-GPR20 antibody A14205, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-05
Question
Will A14205 anti-GPR20 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-04-15
Answer
You can see on the product datasheet, A14205 anti-GPR20 antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-04-15
Question
Is a blocking peptide available for product anti-GPR20 antibody (A14205)?
V. Krishna
Verified customer
Asked: 2018-09-03
Answer
We do provide the blocking peptide for product anti-GPR20 antibody (A14205). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-09-03
Question
We are currently using anti-GPR20 antibody A14205 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
R. Bhatt
Verified customer
Asked: 2013-02-06
Answer
The anti-GPR20 antibody (A14205) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-02-06
