Anti-GPR35 Antibody

GPR35 antibody

Boster Bio Anti-GPR35 Antibody (Catalog# A06269). Tested in WB application(s). This antibody reacts with Mouse, Human.

Product Info Summary

SKU: A06269
Size: 100 µg
Reactive Species: Human, Mouse
Host: Rabbit
Application: WB

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Product Name

Anti-GPR35 Antibody

View all GPR35 Antibodies

SKU/Catalog Number

A06269

Size

100 µg

Form

Liquid

Description

Boster Bio Anti-GPR35 Antibody (Catalog# A06269). Tested in WB application(s). This antibody reacts with Mouse, Human.

Storage & Handling

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Cite This Product

Anti-GPR35 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06269)

Host

Rabbit

Contents

100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A partial length recombinant GPR35 protein (amino acids 173-305)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A06269 is reactive to GPR35 in Human, Mouse

Applications

A06269 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

34.072kDa

Background of GPR35

SmarcAL1 (SWI/SNF-related µatrix-associated actin-dependent regulator of chromatin a-like 1) is also known as HARP, HepA Related Protein, HHARP, SMARCA like Protein 1 and SWI/SNF Related. SmarcAL1 is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein shows sequence similarity to the E. coli RNA polymerase-binding protein HepA. Mutations in this gene are a cause of Schimke immunoosseous dysplasia (SIOD), an autosomal recessive disorder with the diagnostic features of spondyloepiphyseal dysplasia, renal dysfunction, and T-cell immunodeficiency.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot: 2-4 µg/ml

Validation Images & Assay Conditions

Gene/Protein Information For GPR35 (Source: Uniprot.org, NCBI)

Gene Name

GPR35

Full Name

G-protein coupled receptor 35

Weight

34.072kDa

Superfamily

G-protein coupled receptor 1 family

Alternative Names

G protein-coupled receptor 35; GPR35; G-protein coupled receptor 35; KYNA receptor; Kynurenic acid receptor GPR35 G protein-coupled receptor 35 G-protein coupled receptor 35|KYNA receptor|kynurenic acid receptor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GPR35, check out the GPR35 Infographic

GPR35 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GPR35: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-GPR35 Antibody

Question

We are currently using anti-GPR35 antibody A06269 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-30

Answer

The anti-GPR35 antibody (A06269) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-30

Question

Does anti-GPR35 antibody A06269 work on feline WB with brain?

Verified Customer

Verified customer

Asked: 2019-07-24

Answer

Our lab technicians have not validated anti-GPR35 antibody A06269 on feline. You can run a BLAST between feline and the immunogen sequence of anti-GPR35 antibody A06269 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline brain in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-24

Question

Will A06269 anti-GPR35 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-12-06

Answer

It shows on the product datasheet, A06269 anti-GPR35 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-12-06

Question

Our lab want to know about to test anti-GPR35 antibody A06269 on human brain for research purposes, then I may be interested in using anti-GPR35 antibody A06269 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-09-06

Answer

The products we sell, including anti-GPR35 antibody A06269, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-09-06

Question

I was wanting to use your anti-GPR35 antibody for WB for human brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain identification?

A. Singh

Verified customer

Asked: 2017-08-24

Answer

As indicated on the product datasheet, A06269 anti-GPR35 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-08-24

Order DetailsPrice
A06269-100ug

100 μg

$341

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