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Product Info Summary
|Reactive Species:||Human, Mouse|
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Anti-GPR50 Antibody Picoband™
Boster Bio Anti-GPR50 Antibody Picoband™ catalog # A07827-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-GPR50 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07827-2)
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
E.coli-derived human GPR50 recombinant protein (Position: K214-S471).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
A07827-2 is reactive to GPR50 in Human, Mouse
A07827-2 is guaranteed for ELISA, WB Boster Guarantee
Background of GPR50
G protein-coupled receptor 50 is a protein which in humans is encoded by the GPR50 gene. This gene product belongs to the G-protein coupled receptor 1 family. Even though this protein shares similarity with the melatonin receptors, it does not bind melatonin, however, it inhibits melatonin receptor 1A function through heterodimerization. Polymorphic variants of this gene have been associated with bipolar affective disorder in women.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of GPR50 using anti-GPR50 antibody (A07827-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse testis tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPR50 antigen affinity purified polyclonal antibody (Catalog # A07827-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPR50 at approximately 67 kDa. The expected band size for GPR50 is at 67 kDa.
Gene/Protein Information For GPR50 (Source: Uniprot.Org, NCBI)
G-protein coupled receptor 1 family
G protein-coupled receptor 50H9Mel1c; GPR50; H9; melatonin-related receptor; MGC125342; MTR1L GPR50 H9, Mel1c G protein-coupled receptor 50 melatonin-related receptor*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on GPR50, check out the GPR50 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for GPR50: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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