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Product Info Summary
| SKU: | A14949 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-GPR82 Antibody
SKU/Catalog Number
A14949
Size
100μl
Form
Liquid
Description
Boster Bio Anti-GPR82 Antibody catalog # A14949. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GPR82 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14949)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human GPR82. AA range:121-170
Reactive Species
A14949 is reactive to GPR82 in Human, Mouse
Calculated molecular weight
38.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14949 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using GPR82 Polyclonal Antibody
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Western blot analysis of lysates from A549, K562, and HT-29 cells, using GPR82 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-GPR82 Antibody (A14949)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-GPR82 Antibody
Question
Is a blocking peptide available for product anti-GPR82 antibody (A14949)?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
We do provide the blocking peptide for product anti-GPR82 antibody (A14949). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-04-28
Question
We need to test anti-GPR82 antibody A14949 on mouse placenta for research purposes, then I may be interested in using anti-GPR82 antibody A14949 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
The products we sell, including anti-GPR82 antibody A14949, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-26
Question
Is this A14949 anti-GPR82 antibody reactive to the isotypes of GPR82?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
The immunogen of A14949 anti-GPR82 antibody is Synthesized peptide derived from the Internal region of human GPR82. at AA rangle: 90-170. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-18
Question
I was wanting to use your anti-GPR82 antibody for WB for mouse placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse placenta identification?
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
It shows on the product datasheet, A14949 anti-GPR82 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-23
Question
Would anti-GPR82 antibody A14949 work for WB with placenta?
Verified Customer
Verified customer
Asked: 2017-11-24
Answer
According to the expression profile of placenta, GPR82 is highly expressed in placenta. So, it is likely that anti-GPR82 antibody A14949 will work for WB with placenta.
Boster Scientific Support
Answered: 2017-11-24
Question
Will anti-GPR82 antibody A14949 work on horse WB with thyroid?
T. Krishna
Verified customer
Asked: 2014-12-01
Answer
Our lab technicians have not tested anti-GPR82 antibody A14949 on horse. You can run a BLAST between horse and the immunogen sequence of anti-GPR82 antibody A14949 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse thyroid in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-12-01
Question
We are currently using anti-GPR82 antibody A14949 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on canine tissues as well?
H. Johnson
Verified customer
Asked: 2014-03-21
Answer
The anti-GPR82 antibody (A14949) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-03-21
