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Product Info Summary
| SKU: | A30831 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-GPR92 Antibody
SKU/Catalog Number
A30831
Size
100ul
Form
Liquid
Description
Boster Bio Anti-GPR92 Antibody (Catalog# A30831). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GPR92 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30831)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human GPR92. Immunogen sequence location: 241 - 290
Reactive Species
A30831 is reactive to LPAR5 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
41.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30831 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:5000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western Blot analysis of various cells using GPR92 Polyclonal Antibody diluted at 1:500
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Western blot analysis of lysates from HT-29 and K562 cells, using GPR92 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-GPR92 Antibody (A30831)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-GPR92 Antibody
Question
Would anti-GPR92 antibody A30831 work for WB with vagina?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
According to the expression profile of vagina, LPAR5 is highly expressed in vagina. So, it is likely that anti-GPR92 antibody A30831 will work for WB with vagina.
Boster Scientific Support
Answered: 2019-12-06
Question
I have a question about product A30831, anti-GPR92 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30831 anti-GPR92 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-26
Question
Do you have a BSA free version of anti-GPR92 antibody A30831 available?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GPR92 antibody A30831 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-02
Question
I was wanting to use your anti-GPR92 antibody for WB for human vagina on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human vagina identification?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
It shows on the product datasheet, A30831 anti-GPR92 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human vagina in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-29
Question
We are currently using anti-GPR92 antibody A30831 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
The anti-GPR92 antibody (A30831) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-25
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for vagina using anti-GPR92 antibody A30831. Let me know if you need anything else.
V. Jones
Verified customer
Asked: 2019-06-17
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-17
Question
Please see the WB image, lot number and protocol we used for vagina using anti-GPR92 antibody A30831. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-01-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-17
