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Product Info Summary
| SKU: | A30826 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-GPRC5B Antibody
SKU/Catalog Number
A30826
Size
100ul
Form
Liquid
Description
Boster Bio Anti-GPRC5B Antibody (Catalog# A30826). Tested in WB, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GPRC5B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30826)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human GPRC5B. Immunogen sequence location: 61 - 110
Reactive Species
A30826 is reactive to GPRC5B in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
44.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30826 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
ICC/IF 1:200 - 1:1000.
ELISA: 1:5000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunofluorescence analysis of MCF7 cells, using GPRC5B Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from Jurkat, HUVEC, and 293 cells, using GPRC5B Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-GPRC5B Antibody (A30826)
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Customer Q&As
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3 Customer Q&As for Anti-GPRC5B Antibody
Question
We are currently using anti-GPRC5B antibody A30826 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
The anti-GPRC5B antibody (A30826) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-24
Question
Can you help my question with product A30826, anti-GPRC5B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30826 anti-GPRC5B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-12
Question
Does anti-GPRC5B antibody A30826 work on bovine WB with testis?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
Our lab technicians have not validated anti-GPRC5B antibody A30826 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-GPRC5B antibody A30826 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-26
