Anti-Glutathione Peroxidase 1/GPX1 Antibody Picoband®

Glutathione Peroxidase 1/GPX1 antibody

Boster Bio Anti-Glutathione Peroxidase 1/GPX1 Antibody Picoband® catalog # PB9203. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 1 publication(s).

Product Info Summary

SKU: PB9203
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Glutathione Peroxidase 1/GPX1 Antibody Picoband®

View all Glutathione Peroxidase 1/GPX1 Antibodies

SKU/Catalog Number

PB9203

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Glutathione Peroxidase 1/GPX1 Antibody Picoband® catalog # PB9203. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Glutathione Peroxidase 1/GPX1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9203)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human GPX1, different from the related mouse sequence by six amino acids and from the related rat sequence by five amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9203 is reactive to GPX1 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

22 kDa

Calculated molecular weight

22088 MW

Background of Glutathione Peroxidase 1/GPX1

Glutathione peroxidase 1, also known as, GPX-1 is an enzyme that in humans is encoded by the GPX1 gene. It is mapped to 3p21.31. This gene encodes a member of the glutathione peroxidase family, consisting of eight known glutathione peroxidases (Gpx1-8) in humans. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. It has been reported that the protein encoded by this gene protects from CD95-induced apoptosis in cultured breast cancer cells and inhibits 5-lipoxygenase in blood cells, and its overexpression delays endothelial cell growth and increases resistance to toxic challenges. GPX1 is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB9203 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Rat, By Heat

Positive Control

WB: human THP-1 whole cell, rat lung tissue, rat liver tissue, mouse lung tissue, mouse liver tissue
IHC: human colorectal adenocarcinoma tissue, rat lung tissue

Validation Images & Assay Conditions

Gene/Protein Information For GPX1 (Source: Uniprot.org, NCBI)

Gene Name

GPX1

Full Name

Glutathione peroxidase 1

Weight

22088 MW

Superfamily

glutathione peroxidase family

Alternative Names

Glutathione peroxidase 1;GPx-1;GSHPx-1;1.11.1.9;Cellular glutathione peroxidase;GPX1; GPX1 GPXD, GSHPX1 glutathione peroxidase 1 glutathione peroxidase 1|GSHPx-1|cellular glutathione peroxidase|selenoprotein GPX1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GPX1, check out the GPX1 Infographic

GPX1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GPX1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9203 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Ibrolipim attenuates early-stage nephropathy in diet-induced diabetic minipigs: Focus on oxidative stress and fibrogenesis

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5 Customer Q&As for Anti-Glutathione Peroxidase 1/GPX1 Antibody Picoband®

Question

I was wanting to use your anti-Glutathione Peroxidase 1/GPX1 antibody for WB for mouse left adrenal gland on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse left adrenal gland identification?

Verified Customer

Verified customer

Asked: 2019-12-09

Answer

As indicated on the product datasheet, PB9203 anti-Glutathione Peroxidase 1/GPX1 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse left adrenal gland in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-12-09

Question

We are currently using anti-Glutathione Peroxidase 1/GPX1 antibody PB9203 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-11-19

Answer

The anti-Glutathione Peroxidase 1/GPX1 antibody (PB9203) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-19

Question

Does PB9203 anti-Glutathione Peroxidase 1/GPX1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-10-21

Answer

As indicated on the product datasheet, PB9203 anti-Glutathione Peroxidase 1/GPX1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-10-21

Question

Do you have a BSA free version of anti-Glutathione Peroxidase 1/GPX1 antibody PB9203 available?

Verified Customer

Verified customer

Asked: 2018-01-11

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Glutathione Peroxidase 1/GPX1 antibody PB9203 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-01-11

Question

Will anti-Glutathione Peroxidase 1/GPX1 antibody PB9203 work for WB with left adrenal gland?

T. Rodriguez

Verified customer

Asked: 2014-12-12

Answer

According to the expression profile of left adrenal gland, GPX1 is highly expressed in left adrenal gland. So, it is likely that anti-Glutathione Peroxidase 1/GPX1 antibody PB9203 will work for WB with left adrenal gland.

Boster Scientific Support

Answered: 2014-12-12

Order DetailsPrice
PB9203

100μg

$370
PB9203-10ug

10μg sample (liquid)

$99
PB9203-Biotin

100 μg Biotin conjugated

$570
PB9203-Cy3

100 μg Cy3 conjugated

$570
PB9203-Dylight488

100 μg Dylight488 conjugated

$570
PB9203-Dylight550

100 μg Dylight550 conjugated

$570
PB9203-Dylight594

100 μg Dylight594 conjugated

$570
PB9203-FITC

100 μg FITC conjugated

$570
PB9203-HRP

100 μg HRP conjugated

$570
PB9203-APC

100 μg APC conjugated

$670
PB9203-PE

100 μg PE conjugated

$670
PB9203-iFluor647

100 μg iFluor647 conjugated

$670
PB9203-carrier-free

Carrier Free

$370

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PB9203
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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