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Product Info Summary
| SKU: | PB9203 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Glutathione Peroxidase 1/GPX1 Antibody Picoband®
SKU/Catalog Number
PB9203
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Glutathione Peroxidase 1/GPX1 Antibody Picoband® catalog # PB9203. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Glutathione Peroxidase 1/GPX1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9203)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human GPX1, different from the related mouse sequence by six amino acids and from the related rat sequence by five amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9203 is reactive to GPX1 in Human, Mouse, Rat
Observed Molecular Weight
22 kDa
Calculated molecular weight
22.1 kDa
Background of GPX1
Glutathione peroxidase 1, also known as, GPX-1 is an enzyme that in humans is encoded by the GPX1 gene. It is mapped to 3p21.31. This gene encodes a member of the glutathione peroxidase family, consisting of eight known glutathione peroxidases (Gpx1-8) in humans. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. It has been reported that the protein encoded by this gene protects from CD95-induced apoptosis in cultured breast cancer cells and inhibits 5-lipoxygenase in blood cells, and its overexpression delays endothelial cell growth and increases resistance to toxic challenges. GPX1 is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9203 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Rat
Positive Control
WB: human THP-1 whole cell, rat lung tissue, rat liver tissue, mouse lung tissue, mouse liver tissue
IHC: human colorectal adenocarcinoma tissue, rat lung tissue
Validation Images & Assay Conditions
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Western blot analysis of GPX1 using anti-GPX1 antibody (PB9203).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: rat lung tissue lysates,
Lane 3: rat liver tissue lysates,
Lane 4: mouse lung tissue lysates,
Lane 5: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPX1 antigen affinity purified polyclonal antibody (Catalog # PB9203) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPX1 at approximately 22 kDa. The expected band size for GPX1 is at 22 kDa.
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IHC analysis of GPX1 using anti-GPX1 antibody (PB9203).
GPX1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GPX1 Antibody (PB9203) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IHC analysis of GPX1 using anti-GPX1 antibody (PB9203).
GPX1 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GPX1 Antibody (PB9203) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Glutathione Peroxidase 1/GPX1 Antibody Picoband® (PB9203)
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5 Customer Q&As for Anti-Glutathione Peroxidase 1/GPX1 Antibody Picoband®
Question
I was wanting to use your anti-Glutathione Peroxidase 1/GPX1 antibody for WB for mouse left adrenal gland on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse left adrenal gland identification?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
As indicated on the product datasheet, PB9203 anti-Glutathione Peroxidase 1/GPX1 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse left adrenal gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-09
Question
We are currently using anti-Glutathione Peroxidase 1/GPX1 antibody PB9203 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
The anti-Glutathione Peroxidase 1/GPX1 antibody (PB9203) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-19
Question
Does PB9203 anti-Glutathione Peroxidase 1/GPX1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-21
Answer
As indicated on the product datasheet, PB9203 anti-Glutathione Peroxidase 1/GPX1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-21
Question
Do you have a BSA free version of anti-Glutathione Peroxidase 1/GPX1 antibody PB9203 available?
Verified Customer
Verified customer
Asked: 2018-01-11
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Glutathione Peroxidase 1/GPX1 antibody PB9203 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-11
Question
Will anti-Glutathione Peroxidase 1/GPX1 antibody PB9203 work for WB with left adrenal gland?
T. Rodriguez
Verified customer
Asked: 2014-12-12
Answer
According to the expression profile of left adrenal gland, GPX1 is highly expressed in left adrenal gland. So, it is likely that anti-Glutathione Peroxidase 1/GPX1 antibody PB9203 will work for WB with left adrenal gland.
Boster Scientific Support
Answered: 2014-12-12
