Product Info Summary
SKU: | A02637 |
---|---|
Size: | 80 µl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | IHC-P, WB |
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Product info
Product Name
Anti-GPX3 Antibody (Center)
View all Glutathione Peroxidase 3/GPX3 Antibodies
SKU/Catalog Number
A02637
Size
80 µl
Description
Boster Bio Anti-GPX3 Antibody (Center) (Catalog # A02637). Tested in WB, IHC-P application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-GPX3 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02637)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This GPX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-123 amino acids from the Central region of human GPX3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02637 is reactive to GPX3 in Human, Mouse
Reconstitution
Calculated molecular weight
25552 Da
Background of Glutathione Peroxidase 3/GPX3
This gene product belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02637 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
All lanes : Anti-GPX3 Antibody (Center) at 1:1000 dilution
Lane 1: M. plasma lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Observed band size : 22kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
Western blot analysis of lysates from human kidney, plasma, mouse kidney, plasma and rat kidney tissue lysate (from left to right), using GPX3 Antibody (Center) (Cat. #A02637). A02637 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Click image to see more details
GPX3 Antibody (Center) (Cat. #A02637) western blot analysis in mouse kidney tissue lysates (35ug/lane). This demonstrates the GPX3 antibody detected the GPX3 protein (arrow).
Click image to see more details
GPX3 Antibody (Center) (Cat. #A02637)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of GPX3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Protein Target Info & Infographic
Gene/Protein Information For GPX3 (Source: Uniprot.org, NCBI)
Gene Name
GPX3
Full Name
Glutathione peroxidase 3
Weight
25552 Da
Superfamily
glutathione peroxidase family
Alternative Names
Glutathione peroxidase 3, GPx-3, GSHPx-3, Extracellular glutathione peroxidase, Plasma glutathione peroxidase, GPx-P, GSHPx-P, GPX3, GPXP GPX3 GPx-P, GSHPx-3, GSHPx-P glutathione peroxidase 3 glutathione peroxidase 3|GPx-3|extracellular glutathione peroxidase|glutathione peroxidase 3 (plasma)|plasma glutathione peroxidase|selenoprotein GPX3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GPX3, check out the GPX3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GPX3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GPX3 Antibody (Center) (A02637)
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Customer Q&As
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5 Customer Q&As for Anti-GPX3 Antibody (Center)
Question
Is a blocking peptide available for product anti-GPX3/Glutathione Peroxidase 3 antibody (A02637)?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
We do provide the blocking peptide for product anti-GPX3/Glutathione Peroxidase 3 antibody (A02637). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-05-01
Question
I see that the anti-GPX3/Glutathione Peroxidase 3 antibody A02637 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-14
Question
Will anti-GPX3/Glutathione Peroxidase 3 antibody A02637 work on pig IHC with duodenum?
S. Moore
Verified customer
Asked: 2019-05-23
Answer
Our lab technicians have not tested anti-GPX3/Glutathione Peroxidase 3 antibody A02637 on pig. You can run a BLAST between pig and the immunogen sequence of anti-GPX3/Glutathione Peroxidase 3 antibody A02637 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig duodenum in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-23
Question
We are currently using anti-GPX3/Glutathione Peroxidase 3 antibody A02637 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-04-24
Answer
The anti-GPX3/Glutathione Peroxidase 3 antibody (A02637) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-04-24
Question
Here is the WB image, lot number and protocol we used for fetal liver placenta using anti-GPX3/Glutathione Peroxidase 3 antibody A02637. Please let me know if you require anything else.
A. Li
Verified customer
Asked: 2013-09-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-09-20