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GPX4 antibody (M02059) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 59 Publications

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Immunofluorescent analysis using the Antibody at 1:500 dilution.

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Anti-GPX4 Rabbit Monoclonal Antibody

Name: Anti-GPX4 Rabbit Monoclonal Antibody
Brand: Boster Bio
SKU: M02059
Price: 370 USD
Availability: InStock
Rating: 100 (2 reviews)

GPX4 antibody

59 Publications|

2 Reviews

|Submit a Review

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	IF, IHC, ICC, WB

Tested Applications

GPX4 antibody was positively detected by WB in:

mouse RAW264.7(WT) whole cell, mouse RAW264.7(KO) whole cell, human MCF-7 whole cell, human HepG2 whole cell, human CACO-2 whole cell, human 293T whole cell, human K562 whole cell, rat PC-12 whole cell, rat NRK whole cell, mouse RAW264.7 whole cell, mouse NIH/3T3 whole cell, rat kidney tissue, rat testis tissue, mouse kidney tissue, mouse testis tissue, human HCT116- WT whole cell, human HCT116-GPX4 KO whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ICC

Mouse

Tested

Recommend

Human

Tested

Recommend

Rat

Tested

Recommend

Product Information

Product Name

Anti-GPX4 Rabbit Monoclonal Antibody
View all GPX4 Antibodies

Form

Liquid

Uniprot

P36969

Gene ID

2879

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

ACCO-7

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human GPX4

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

19 kDa

Calculated Molecular Weight

22.2 kDa

Cite This Product

Anti-GPX4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02059)

Protocols

Western Blot protocol for GPX4 antibody

Review Protocol

Publications

EP300 drives the progression of oral squamous cell carcinoma by regulating NUPR1-mediated ferroptosis and EMT

Published: 2026-08-26

Journal: CLINICAL AND EXPERIMENTAL MEDICINE

Application: WB

Impact Factor: 4.50

Species: Human,Mouse

SKU	M02059
Application	Western Blot
Sample	PC-12 (rat adrenal pheochromocytoma cells, highly differentiated)
Sample Processing Description	① Normal PC12 cells (rat adrenal pheochromocytoma cells, highly differentiated)② PC12 cells are treated with β-amyloid (Aβ) to establish an in vitro Alzheimer's disease (AD) model.③ The AD in vitro model is treated with the therapeutic drug.
Other Reagents	RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody	GPX4 Rabbit Monoclonal Antibody
Primary Incubation	1:5000, overnight at 4 ℃
Secondary Antibody	HRP-conjugated goat anti-rabbit IgG
Secondary Incubation	1:10000, 1 hour in RT
Detection	Substrate: ECL substrate; Image system: ChemiDoc MP
Results Summary	① Normal PC-12 cells (rat adrenal pheochromocytoma cells (highly differentiated)) ② PC12 cells were induced with β-amyloid protein (Aβ) to establish an in vitro model of Alzheimer's disease (AD) ③ The AD in vitro model was treated with therapeutic drugs. Experimental results showed that the target band was clear, the total GPX4 protein expression was downregulated after modeling, and the expression level rebounded after drug treatment.

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Free Secondary Antibody

Anti-GPX4 Rabbit Monoclonal Antibody

GPX4 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

EP300 drives the progression of oral squamous cell carcinoma by regulating NUPR1-mediated ferroptosis and EMT

Sodium butyrate alleviates neuronal ferroptosis after gas explosion-induced traumatic brain injury via regulation of JNK/P38 MAPK signaling pathway

Polygonatum odoratum extract ameliorates acetaminophen-induced acute liver injury by modulating the gut-liver axis and remodeling microbial and ceramide metabolism to suppress hepatic ferroptosis: insights from multi-omics

Tetrastigma hemsleyanum Diels & Gilg ameliorates alcohol-associated liver disease by modulating the gut-liver axis and ferroptosis

Nonpolysaccharide fraction of Lonicerae japonicae Flos attenuates cyclophosphamide-induced immunosuppression associated with modulation of the Keap1/Nrf2/HO-1/GPX4 signalling pathway

Isoliquiritigenin ameliorates Pseudomonas aeruginosa-induced acute lung injury through inhibiting lung epithelial cell ferroptosis via PPARγ/Nrf2/GPX4 axis

SPOCK1 acts as a negative regulator of ferroptosis in non-small cell lung cancer

Lycorine Suppresses DPP3-Mediated Nrf2 Signaling, Thereby Reducing SLC7A11 Levels to Trigger Ferroptosis in Colorectal Cancer Cells

Di-(2-ethylhexyl) phthalate induces spermatogenic dysfunction via p38/NCOA4-dependent ferritinophagy and ferroptosis in Sertoli cells

Retrorsine Induces Hepatotoxicity through ATF3-Mediated Ferroptosis in Mouse Primary Hepatocytes and CYP3A4-HepG2 Cells

IER3 Promotes Non-Small Cell Lung Cancer Malignancy by Suppressing Ferroptosis via the AKT/GSK3β/NRF2 Pathway

Iterative-Designed Multifunctional Carbon Dots Nanodrug for Celastrol Loading, Targeting Delivery, and Combination Therapy of Kidney Stones

Chaihu Guizhi Ganjiang decoction attenuates chronic pancreatitis by suppressing acinar cell ferroptosis via regulating p53/SLC7A11/GPX4 pathway

Mulberry leaf flavonoid extract inhibits high glucose-induced ferroptosis by regulating the PI-3K/Akt/Nrf2/GPX4 pathway in HT22 mouse hippocampal neuronal cells

Andrographolide Promotes Ferroptosis in Pancreatic Cancer via Targeting and Activating HSP90/GPX4 Ubiquitination

IL-22 attenuates MAFLD progression by modulating oxidative stress and ferroptosis

Gambogic acid restores wild-type-like p53 functions to induce ferroptosis in mutant p53 cancer cells

An Integrated Approach of experiment validation and Single-cell RNA sequencing reveal dual-pathway inhibition of NLRP3/ferroptosis by flavonoids of Sanyeqing fibrous root in acute lung injury

Muscone attenuates myocardial infarction by regulating ferroptosis of cardiomyocytes through Nrf2/system Xc-/GPX4 signaling pathway

Vitamin C alleviates PM2.5-induced thyroid injury and ferroptosis by regulating the IL-17A/IL-17RA/ACT1 signaling pathway

Microwave exposure induced ferroptosis by inhibiting the Nrf2 pathway and affected reproductive function in male mice

Celastrol Attenuates Ferroptosis-Mediated Intestinal Ischemia/Reperfusion-Induced Acute Lung Injury via Hippo-YAP Signaling

Schisandrin B ameliorates Alzheimer's disease by suppressing neuronal ferroptosis and ensuing microglia M1 polarization

Echinococcus granulosus-Induced Liver Damage Through Ferroptosis in Rat Model

Thonningianin A ameliorates acetaminophen-induced liver injury by activating GPX4 and modulating endoplasmic reticulum stress

Inhibition of ferroptosis counteracts the advanced maternal age-induced oocyte deterioration

The mutualistic relationship between M2c macrophages of TGFβ1 induction and gastric cancer cells: the correlation between protective mechanisms in the tumor microenvironment and polarization of subtypes of cells

MT1G promotes iron autophagy and inhibits the function of gastric cancer cell lines by intervening in GPX4/SQSTM1

Triptolide induces hepatotoxicity by promoting ferroptosis through Nrf2 degradation

Sirtuin1 Suppresses Calcium Oxalate Nephropathy via Inhibition of Renal Proximal Tubular Cell Ferroptosis Through PGC-1α-mediated Transcriptional Coactivation

Genistein Induces Ferroptosis in Colorectal Cancer Cells via FoxO3/SLC7A11/GPX4 Signaling Pathway

Stachyose ameliorates myocardial ischemia–reperfusion injury by inhibiting cardiomyocyte ferroptosis and macrophage pyroptosis

Se-Methylselenocysteine Ameliorates DEHP-Induced Ferroptosis in Testicular Sertoli Cells via the Nrf2/GPX4 Axis

PTEN Deficiency Induced by Extracellular Vesicle miRNAs from Clonorchis sinensis Potentiates Cholangiocarcinoma Development by Inhibiting Ferroptosis

Cardiac-targeted and ROS-responsive liposomes containing puerarin for attenuating myocardial ischemia-reperfusion injury

Superenhancer-driven circRNA Myst4 involves in pulmonary artery smooth muscle cell ferroptosis in pulmonary hypertension

SIRT5 safeguards against T-2 toxin induced liver injury by repressing iron accumulation, oxidative stress, and the activation of NLRP3 inflammasome

Endonuclear Circ-calm4 regulates ferroptosis via a circR-Loop of the COMP gene in pulmonary artery smooth muscle cells

Contribution of impaired autophagy, mitochondrial dysfunction and abnormal lipolysis to epididymal aging in mice

Expression of the laminin genes family and its relationship to prognosis in pancreatic carcinoma

Liproxstatin-1 attenuates acute hypertriglyceridemic pancreatitis through inhibiting ferroptosis in rats

Mitochondria-targeting peptide SS-31 attenuates ferroptosis via inhibition of the p38 MAPK signaling pathway in the hippocampus of epileptic rats

A multifunctional bimetallic nanoplatform for enhancing radiotherapy via oxidative damage and ferroptosis of cervical cancer

Characterization of PKCα-rutin interactions and its application as a treatment strategy for PAH by inhibiting ferroptosis

A novel UVA-associated circUBE2I mediates ferroptosis in HaCaT cells

Naotaifang formula attenuates OGD/R-induced inflammation and ferroptosis by regulating microglial M1/M2 polarization through BMP6/SMADs signaling pathway

Neuroprotective effects of salidroside against 6-OHDA-induced oxidative stress in PC12 cells

Paeoniae Radix Rubra extract attenuates cerebral ischemia injury by inhibiting ferroptosis and activating autophagy through the PI3K/Akt signalling pathway

Andrographolide induced ferroptosis in multiple myeloma cells by regulating the P38/Nrf2/HO-1 pathway

Protectin conjugates in tissue regeneration 1 alleviates sepsis-induced acute lung injury by inhibiting ferroptosis

Ferroptosis is involved in corpus cavernosum smooth muscle cells impairment in diabetes mellitus-induced erectile dysfunction

Levistilide a Induces Ferroptosis by Activating the Nrf2/HO-1 Signaling Pathway in Breast Cancer Cells

Dexpramipexole Attenuates White Matter Injury to Facilitate Locomotion and Motor Coordination Recovery via Reducing Ferroptosis after Intracerebral Hemorrhage

Solasonine Causes Redox Imbalance and Mitochondrial Oxidative Stress of Ferroptosis in Lung Adenocarcinoma

Cordycepin alleviated metabolic inflammation in Western diet-fed mice by targeting intestinal barrier integrity and intestinal flora

Multienzyme-like Reactivity Cooperatively Impairs Glutathione Peroxidase 4 and Ferroptosis Suppressor Protein 1 Pathways in Triple-Negative Breast Cancer for Sensitized Ferroptosis Therapy

Autophagy modulation in resveratrol protective effects on steroidogenesis in high-fat diet-fed mice and H2O2-challenged TM3 cells

GPX4-Regulated Ferroptosis Mediates S100-Induced Experimental Autoimmune Hepatitis Associated with the Nrf2/HO-1 Signaling Pathway

A novel strategy for glioblastoma treatment by induction of noptosis, an NQO1-dependent necrosis

Review

Have you used Anti-GPX4 Rabbit Monoclonal Antibody?

2 Reviews For Anti-GPX4 Rabbit Monoclonal Antibody

Anti-GPX4 Antibody (M02059) delivered crisp target bands at the expected molecular mass with minimal background. Western blot analysis indicated diminished GPX4 abundance in Aβ-treated PC-12 AD model cells, while subsequent drug administration rescued its

The GPX4 antibody was used to detect the expression of the protein in mouse uterine tissue. The WB results showed clear bands, and the antibody could be reused after recovery with good performance, offering excellent cost-effectiveness.

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