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GPX4 antibody (A02059-1) performs well in ELISA, IF, IHC, WB and shows reactivity with Human, Monkey, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 17 Publications

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a02059 1 gpx4 primary antibodies fcm testing 1

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Flow Cytometry analysis of THP-1 cells using anti-Glutathione Peroxidase 4/GPX4 antibody (A02059-1).
Overlay histogram showing THP-1 cells stained with A02059-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Glutathione Peroxidase 4/GPX4 Antibody (A02059-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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Anti-Glutathione Peroxidase 4/GPX4 Antibody Picoband®

Name: Anti-Glutathione Peroxidase 4/GPX4 Antibody Picoband®
Brand: Boster Bio
SKU: A02059-1
Price: 370 USD
Availability: InStock
Rating: 100 (1 reviews)

GPX4 antibody

17 Publications|

1 Review

|Submit a Review

Reactive Species	Host	Application
Human, Monkey, Mouse, Rat	Rabbit	ELISA, IF, IHC, WB

Tested Applications

GPX4 antibody was positively detected by WB in:

human HepG2 whole cell, human MCF-7 whole cell, human RT4 whole cell, monkey COS-7 whole cell, rat kidney tissue, rat testis tissue, mouse kidney tissue, human HCT116- WT whole cell, human HCT116-GPX4 KO whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

GPX4 antibody was positively detected by IHC in:

human testis tissue, mouse testis tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

GPX4 antibody was positively detected by FC in:

THP-1 cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ELISA

Human

Tested

Recommend

Monkey

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Recommend

Bovine

Predicted

Sheep

Predicted

Dog

Predicted

Product Information

Product Name

Anti-Glutathione Peroxidase 4/GPX4 Antibody Picoband®
View all GPX4 Antibodies

Form

Lyophilized

Uniprot

P36969

Gene ID

2879

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Glutathione Peroxidase 4/GPX4 recombinant protein (Position: A30-F197).

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Observed Molecular Weight

19 kDa

Calculated Molecular Weight

22.2 kDa

Background of GPX4

Glutathione peroxidase 4, also known as GPX4, is an enzyme that in humans is encoded by the GPX4 gene. This gene encodes a member of the glutathione peroxidase protein family. Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. The encoded protein has been identified as a moonlighting protein based on its ability to serve dual functions as a peroxidase as well as a structural protein in mature spermatozoa. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified. Alternative splicing results in multiple transcript variants.

Cite This Product

Anti-Glutathione Peroxidase 4/GPX4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02059-1)

Protocols

Western Blot protocol for GPX4 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for GPX4 antibody

Review Protocol

Flow Cytometry (FC) protocol for GPX4 antibody

Review Protocol

Publications

SGLT2 inhibitors ameliorate myocardial hypertrophy in rats via AMPK/Nrf2-mediated ferroptosis suppression and antioxidant response

Published: 2026-05-28

Journal: EUROPEAN JOURNAL OF PHARMACOLOGY

Application: WB

Impact Factor: 5.70

Species: Rat

PMID: 42214780

SKU	A02059-1
Application	Western Blot
Sample	Liver tissue from an ANIT-induced rat model
Sample Processing Description	Cholestatic liver injury was induced in SD rats via intragastric administration of ANIT. Liver tissues were harvested 48 hours later, and proteins were extracted using RIPA lysis buffer. Following BCA quantification, the samples were mixed with loading buffer and boiled for denaturation.
Other Reagents	Blocking buffer, RIPA lysis buffer
Primary Antibody	COL6A1 Antibody Picoband®
Primary Incubation	Glutathione Peroxidase 4/GPX4 Antibody Picoband® GPX4 antibody
Secondary Antibody	HRP-conjugated anti-rabbit IgG secondary antibody
Secondary Incubation	1:50000
Detection	ECL, Imaging system: Contact-Based Non-Destructive Signal Imaging
Results Summary	The image shows the following lanes: lanes 1 and 4 represent liver samples from control group rats, displaying clear and uniform GPX4 bands; lanes 2 and 5 represent the ANIT-treated group; and lanes 3 and 6 represent the drug-treated group, showing markedly increased GPX4 bands. These results demonstrate that the antibody effectively detects changes in GPX4 expression.

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Anti-Glutathione Peroxidase 4/GPX4 Antibody Picoband®

GPX4 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

SGLT2 inhibitors ameliorate myocardial hypertrophy in rats via AMPK/Nrf2-mediated ferroptosis suppression and antioxidant response

A polysaccharide from Pueraria lobata ameliorates hepatic fibrosis via gut microbiota-dependent suppression of ferroptosis

Sinapic acid and glabridin synergistically mitigate androgenetic alopecia by modulating ferroptosis through stabilization of β-catenin against ubiquitin-dependent degradation

IDO1-mediated AhR activation up-regulates pentose phosphate pathway via NRF2 to inhibit ferroptosis in lung cancer

Phytochemical Composition and Skin-Friendly Activities of the Ethyl Acetate Fraction in Ophioglossum vulgatum Linn., an In Vitro Study

Exploring the mechanism of Ling-Gui-Zhu-Gan decoction in metabolic cardiomyopathy via inhibiting ferroptosis

MPV17 Prevents Myocardial Ferroptosis and Ischemic Cardiac Injury through Maintaining SLC25A10-Mediated Mitochondrial Glutathione Import

SIRT5 safeguards against T-2 toxin induced liver injury by repressing iron accumulation, oxidative stress, and the activation of NLRP3 inflammasome

Contribution of impaired autophagy, mitochondrial dysfunction and abnormal lipolysis to epididymal aging in mice

Expression of the laminin genes family and its relationship to prognosis in pancreatic carcinoma

Mitochondria-targeting peptide SS-31 attenuates ferroptosis via inhibition of the p38 MAPK signaling pathway in the hippocampus of epileptic rats

New insights into the spleen injury by mitochondrial dysfunction of chicken under polystyrene microplastics stress

Shikonin promotes ferroptosis in HaCaT cells through Nrf2 and alleviates imiquimod-induced psoriasis in mice

Maresin conjugates in tissue regeneration-1 suppresses ferroptosis in septic acute kidney injury

Proanthocyanidin promotes functional recovery of spinal cord injury via inhibiting ferroptosis

Anti-adipogenesis and antioxidative defense status of a crude extract of ‘Kalasin 2’ peanut sprouts in 3T3-L1 mouse adipocytes

Cloning, characterization, and expression analysis of goat (Capra hircus) phospholipid hydroperoxide glutathione peroxidase (PHGPx)

Review

Have you used Anti-Glutathione Peroxidase 4/GPX4 Antibody Picoband®?

1 Review For Anti-Glutathione Peroxidase 4/GPX4 Antibody Picoband®

Anti-GPX4 Antibody (A02059-1) reliably detected the upregulation of GPX4 in liver tissues from ANIT-induced cholestatic rats by WB, with only faint non-specific bands observed.

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