Anti-GRASP65 GORASP1 Antibody

Product Name

Anti-GRASP65 GORASP1 Antibody

View all GRASP65 Antibodies

SKU/Catalog Number

A05896

Size

100ul

Form

Liquid

Description

Boster Bio Anti-GRASP65 GORASP1 Antibody catalog # A05896. Tested in WB,ICC,IF,IHC,Flow Cytometry,IP applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GRASP65 GORASP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05896)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human BMP-5.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05896 is reactive to Gorasp1 in Human

Applications

A05896 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

47.673kDa

Background of GRASP65

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Required for the integrity and for normal function of the Golgi apparatus and the trans-Golgi network. Plays a role in insulin-stimulated translocation of GLUT4 to the cell membrane. Plays a role in M6PR transport from the trans-Golgi network to endosomes. Plays a role in the internalization of EGFR from the cell membrane into endosomes. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis.

Chen D., Gene 174:129-134(1996) [PubMed: 8863739].
Bao X.,Eur. J. Biochem. 269:259-271(2002) [PubMed: 11784320].
Ota T., Nat. Genet. 36:40-45(2004) [PubMed: 14702039].<

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1,000
ICC: 1:50-1:200
IHC: 1:50-1:200
FC: 1:50-1:100

Validation Images & Assay Conditions

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Figure 2. Immunocytochemistry staining of GORASP1 using Anti-GRASP65 GORASP1 Antibody (A05896).

ICC staining GRASP65 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde

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Figure 1. Western blotting validation for Anti-GRASP65 GORASP1 Antibody A05896

Western blot analysis of GRASP65 on different cell lysates using anti-GRASP65 at 1/500 dilution.
Positive control：
Line 1: THP-1
Line 2: SKBR3
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For Gorasp1 (Source: Uniprot.org, NCBI)

Alternative Names

Golgi peripheral membrane protein p65; Golgi phosphoprotein 5MGC118897; golgi reassembly stacking protein 1, 65kDa; Golgi reassembly-stacking protein 1; Golgi reassembly-stacking protein of 65 kDa; GRASP65MGC118894; P6565 kDa Gorasp1|GRASP65|golgi reassembly stacking protein 1|Golgi reassembly-stacking protein 1|golgi peripheral membrane protein p65|golgi reassembly-stacking protein of 65 kDa

For more info on Gorasp1, check out the Gorasp1 Infographic

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In this infographic, you will see the following information for Gorasp1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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